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Alexa Fluor® 700 Rat IgG2a Isotype Control

 
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Contents: Alexa Fluor® 700 Rat IgG2a Isotype Control
Catalog Number: 56-4321
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Isotype: Rat IgG2a, κ

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56-4321-80 25 ug


   

Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
14-4321 Affinity Purified Rat IgG2a Isotype Control N/A N/A FC 
16-4321 Functional Grade* Purified Rat IgG2a Isotype Control N/A N/A FC 
13-4321 Biotin Rat IgG2a Isotype Control N/A N/A FC 
11-4321 FITC Rat IgG2a Isotype Control 488 518 FC 
12-4321 PE Rat IgG2a Isotype Control 488 575 FC 
15-4321 PE-Cy5 Rat IgG2a Isotype Control 488 670 FC 
17-4321 APC Rat IgG2a Isotype Control 633 660 FC 
25-4321 Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2a Isotype Control 488 760 FC 
35-4321 PE-Cy5.5 Rat IgG2a Isotype Control 488 690 FC 
72-4321 Phycoerythrin (PE) Set Rat IgG2a Isotype Control 488 575 IC Flow 
51-4321 Alexa Fluor® 647 Rat IgG2a Isotype Control 633 668 FC 
53-4321 Alexa Fluor® 488 Rat IgG2a Isotype Control 488 519 FC 
56-4321 Alexa Fluor® 700 Rat IgG2a Isotype Control (APC-Cy5.5 Equivalent) 633 723 FC 
57-4321 Pacific Blue® Rat IgG2a Isotype Control 402 421 FC 
27-4321 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2a Isotype Control (APC-Cy7 Equivalent) 633 779 FC 
45-4321 PerCP-Cy5.5 Rat IgG2a Isotype Control 488 690 FC 
93-4321 eFluorTM 605NC Rat IgG2a Isotype Control 350-500 605 FC 
94-4321 eFluorTM 625NC Rat IgG2a Isotype Control 350-500 625 FC 
95-4321 eFluorTM 650NC Rat IgG2a Isotype Control 350-500 650 FC 
47-4321 Allophycocyanin-eFluorTM 780 Rat IgG2a Isotype Control (APC-Alexa Fluor® 750 replacement) 633 780 FC 
48-4321 eFluorTM 450 Rat IgG2a Isotype Control (Pacific Blue® replacement) 405 450 FC 
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.


Description


The monoclonal rat IgG2a, κ is useful as an isotype control immunoglobulin.


Applications Reported


For research use only, not for diagnostic or therapeutic use. Alexa Fluor® 700 Rat IgG2a Iso Cntrl has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.


Applications Tested


This rat IgG2a isotype control has been tested by flow cytometric analysis of mouse splenocyte suspension. This can be used at the same concentration as the experimental antibody.


Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.


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