Product Information

Contents: Alexa Fluor® 700 anti-mouse CD117 (c-Kit, cKit) - neutralizing
Catalog Number: 56-1172
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: ACK2
Isotype: Rat IgG2b, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-1172	PE anti-mouse CD117 (c-Kit, cKit)	488	575	FC
14-1172	Affinity Purified anti-mouse CD117 (c-Kit, cKit) - neutralizing	N/A	N/A	FA FC IH/F IP WB
15-1172	PE-Cy5 anti-mouse CD117 (c-Kit, cKit)	488	670	FC
16-1172	Functional Grade* Purified anti-mouse CD117 (c-Kit, cKit) - neutralizing	N/A	N/A	FA FC
17-1172	APC anti-mouse CD117 (c-Kit, cKit)	633	660	FC
25-1172	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD117 (c-Kit, cKit)	488	760	FC
27-1172	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD117 (c-Kit, cKit) (APC-Cy7 Equivalent)	633	779	FC
56-1172	Alexa Fluor® 700 anti-mouse CD117 (c-Kit, cKit) (APC-Cy5.5 Equivalent)	633	723	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The ACK2 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 (Thy1^lo) and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells. ACK2 has been reported to be a blocking antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use.

Applications Tested

This ACK2 antibody has been tested by flow cytometric analysis of bone marrow suspension cells. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Rico-Vargas, S., et al. 1994. c-kit expresssion by B cell precursors in mouse bone marrow. J. Immunol. 152: 2845.

Ito M, Kawa Y, et al. 1999. Removal of stem cell factor or addition of monoclonal anti-c-KIT antibody induces apoptosis in murine melanocyte precursors. J Invest Dermatol. 112: 796-801.

Vincent S, Segretain D, et al. 1998. Stage-specific expression of the Kit receptor and its ligand (KL) during male gametogenesis in the mouse: a Kit-KL interaction critical for meiosis. Development. 125: 4585-93.

Sato D, Lai ZF, et al. 1996. Impairment of Kit-dependent development of interstitial cells alters contractile responses of murine intestinal tract. Am J Physiol. 271: G762-71.

Yoshida H, Kunisada T, et al. 1996. Distinct stages of melanocyte differentiation revealed by anlaysis of nonuniform pigmentation patterns. Development. 122: 1207-14.

Yoshinaga K, Nishikawa S, et al. 1991. Role of c-kit in mouse spermatogenesis: identification of spermatogonia as a specific site of c-kit expression and function. Development. 113:689-99.

Torihashi S, Ward SM, et al. 1995. c-kit-dependent development of interstitial cells and electrical activity in the murine gastrointestinal tract. Cell Tissue Res. 280:97-111.

Feng H, Sandlow JI, Sandra A. 1997. Expression and function of the c-kit proto-oncogene protein in mouse sperm. Biol Reprod. 57:194-203.

Feng H, Sandlow JI, Sandra A. 1998. The c-kit receptor and its possible signaling transduction pathway in mouse spermatozoa. Mol Reprod Dev. 49:317-26.

Prasanth SG and Ali S. 2003. Expression of proto-oncogene c-kit receptor in rats (Rattus norvegicus) and identification of a mutant mRNA transcript implicated in spermatogenic failure. DNA Cell Biol. 2003 Jul;22(7):447-56. (ACK2, rat cross-reactivity, PubMed)

Ismail RS, Okawara Y, et al. 1996. Hormonal regulation of the ligand for c-kit in the rat ovary and its effects on spontaneous oocyte meiotic maturation. Mol Reprod Dev. 1996 Apr;43(4):458-69. (ACK2, rat cross-reactivity and blocking, PubMed)

Kim HM, Shin HY, and Lee EH. 1998. Morphological alterations in rat peritoneal mast cells by stem cell factor. Immunology. 1998 Jun;94(2):242-6. (ACK2, rat cross-reactivity and blocking, PubMed)

Czechowicz A, Kraft D, Weissman IL, and Bhattacharya D. 2007. Efficient transplantation via antibody-based clearance of hematopoietic stem cell niches. Science. 23;318(5854):1296-9. (ACK2, in vivo depletion HSC, PubMed)

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Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.