Product Information

Contents: Alexa Fluor® 700 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)
Catalog Number: 56-0621
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MEL-14
Isotype: Rat IgG2a, κ

Staining of BALB/c splenocytes with 0.03 μg of Alexa Fluor® 700 Rat IgG2a Iso Cntrl (cat. 56-4321) (open histogram) or 0.03 μg of Alexa Fluor® 700 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0621	Affinity Purified anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	N/A	N/A	FA FC IHC IP
16-0621	Functional Grade* Purified anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	N/A	N/A	FA FC
13-0621	Biotin anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	N/A	N/A	FC
11-0621	FITC anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	488	518	FC
12-0621	PE anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	488	575	FC
15-0621	PE-Cy5 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	488	670	FC
17-0621	APC anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	633	660	FC
25-0621	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	488	760	FC
56-0621	Alexa Fluor® 700 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) (APC-Cy5.5 Equivalent)	633	723	FC
57-0621	Pacific Blue® anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	402	421	FC
45-0621	PerCP-Cy5.5 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) (PE-Cy5.5 replacement)	488	690	FC
93-0621	eFluor^TM 605^NC anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	350-500	605	FC
47-0621	Allophycocyanin-eFluor^TM 780 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) (APC-Alexa Fluor® 750 replacement)	633	780	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MEL-14 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Gallatin, W. M., I. L. Weissman, et al. (1983). "A cell-surface molecule involved in organ-specific homing of lymphocytes." Nature 304(5921): 30-4.
Siegelman, M. H., I. C. Cheng, et al. (1990). "The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding." Cell 61(4): 611-22.

Related Products

Cat. 56-4321 Alexa Fluor® 700 Rat IgG2a Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.