Product Information

Contents: Alexa Fluor® 700 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)
Catalog Number: 56-0454
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 104
Isotype: Mouse IgG2a, κ

Staining of BALB/c splenocytes with 0.25 μg of Alexa Fluor® 700 Mouse IgG2a, K Iso Cntrl (cat. 56-4724) (open histogram) or 0.25 μg of Alexa Fluor® 700 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0454	FITC anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	488	518	FC
12-0454	PE anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	488	575	FC
13-0454	Biotin anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	N/A	N/A	FC IH/F
14-0454	Affinity Purified anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	N/A	N/A	FC IH/F IP
17-0454	Allophycocyanin (APC) anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	633	660	FC
27-0454	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (APC-Cy7 Equivalent)	633	779	FC
35-0454	PE-Cy5.5 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	488	690	FC
45-0454	PerCP-Cy5.5 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2)	488	690	FC
56-0454	Alexa Fluor® 700 anti-mouse CD45.2 (C57BL/6, Balb/c, old Ly5.1, new Ly5.2) (APC-Cy5.5 Equivalent)	633	723	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 104 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This 104 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Shen, F-W. (1981). "Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. In Monoclonal antibodies and T cell Hybridomas; Perspective and technical advances, eds. Hammerling, G.J., U. Hammerling, and J.F. Kearney": 25-31.

Related Products

Cat. 56-4724 Alexa Fluor® 700 Mouse IgG2a, K Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.