Product Information

Contents: Alexa Fluor® 700 anti-mouse/human CD44 (Pgp-1, Ly-24)
Catalog Number: 56-0441
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: IM7
Isotype: Rat IgG2b, κ

Staining of BALB/c splenocytes with 0.03 μg of Alexa Fluor® 700 Rat IgG2a Iso Cntrl (cat. 56-4321) (open histogram) or 0.03 μg of Alexa Fluor® 700 anti-mo/hu CD44 (IM7) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0441	Affinity Purified anti-mouse/human CD44 (Pgp-1, Ly-24)	N/A	N/A	FA FC IHC IP WB
16-0441	Functional Grade* Purified anti-mouse/human CD44 (Pgp-1, Ly-24)	N/A	N/A	FA FC
13-0441	Biotin anti-mouse/human CD44 (Pgp-1, Ly-24)	N/A	N/A	FC
11-0441	FITC anti-mouse/human CD44 (Pgp-1, Ly-24)	488	518	FC
12-0441	PE anti-mouse/human CD44 (Pgp-1, Ly-24)	488	575	FC
15-0441	PE-Cy5 anti-mouse/human CD44 (Pgp-1, Ly-24)	488	670	FC
17-0441	APC anti-mouse/human CD44 (Pgp-1, Ly-24)	633	660	FC
25-0441	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse/human CD44 (Pgp-1, Ly-24)	488	760	FC
35-0441	PE-Cy5.5 anti-mouse/human CD44 (Pgp-1, Ly-24)	488	690	FC
56-0441	Alexa Fluor® 700 anti-mouse/human CD44 (Pgp-1, Ly-24) (APC-Cy5.5 Equivalent)	633	723	FC
57-0441	Pacific Blue® anti-mouse/human CD44 (Pgp-1, Ly-24)	402	421	FC
27-0441	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse/human CD44 (Pgp-1, Ly-24) (APC-Cy7 Equivalent)	633	779	FC
45-0441	PerCP-Cy5.5 anti-mouse/human CD44 (Pgp-1, Ly-24)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This IM7 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Trowbridge, I. S., J. Lesley, et al. 1982.Biochemical characterization and cellular distribution of a polymorphic, murine cell-surface glycoprotein expressed on lymphoid tissues. Immunogenetics 15(3): 299-312.
Lesley, J. and I. S. Trowbridge 1982. Genetic characterization of a polymorphic murine cell-surface glycoprotein. Immunogenetics 15(3): 313-20.
Maiti A, Maki G, Johnson P. TNF-alpha induction of CD44-mediated leukocyte adhesion by sulfation. Science. 1998. Oct 30;282(5390):941-3.

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.