human CD38

Contents: Alexa Fluor® 700 anti-human CD38
Catalog Number: 56-0389
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIT2
Isotype: Mouse IgG1, κ
HLDA No.: III 155

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
56-0389-71	25 tests	$145
56-0389-73	100 tests	$295

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with 0.5 μg of Alexa Fluor® 700 Mouse IgG1, K Iso Cntrl (cat. 56-4714) (open histogram) or Alexa Fluor® 700 anti-human CD38 (HIT2) (colored histogram). Cells in the lymphocyte (left) and monocyte (right) gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0389	Affinity Purified anti-human CD38	N/A	N/A	FC IHC
13-0389	Biotin anti-human CD38	N/A	N/A	FC
11-0389	FITC anti-human CD38	488	518	FC
12-0389	PE anti-human CD38	488	575	FC
15-0389	PE-Cy5 anti-human CD38	488	670	FC
17-0389	Allophycocyanin (APC) anti-human CD38	633	660	FC
25-0389	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD38	488	760	FC
56-0389	Alexa Fluor® 700 anti-human CD38	633	723	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This HIT2 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This HIT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Joshua, D., A. Petersen, et al. (1996). "The labelling index of primitive plasma cells determines the clinical behaviour of patients with myelomatosis." Br J Haematol 94(1): 76-81.
Horenstein, A. L., H. Stockinger, et al. (1998). "CD38 binding to human myeloid cells is mediated by mouse and human CD31." Biochem J 330(Pt 3): 1129-35.
Deaglio, S., M. Morra, et al. (1998). "Human CD38 (ADP-ribosyl cyclase) is a counter-receptor of CD31, an Ig superfamily member." J Immunol 160(1): 395-402.

Related Products

Cat. 56-0381 Alexa Fluor® 700 anti-mouse CD38 (APC-Cy5.5 Equivalent) (clone 90)
Cat. 56-4714 Alexa Fluor® 700 Mouse IgG1, K Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.