Product Information

Contents: Alexa Fluor® 700 anti-mouse CD38
Catalog Number: 56-0381
Sizes: 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 90
Isotype: Rat IgG2a, κ

Staining of BALB/c splenocytes with FITC anti-mouse CD3e (145-2C11) (cat. 11-0031) and 0.06 μg of Alexa Fluor® 700 Rat IgG2a Iso Cntrl (cat. 56-4321) (left) or 0.06 μg of Alexa Fluor® 700 anti-mouse CD38 (90) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0381	Affinity Purified anti-mouse CD38	N/A	N/A	FA FC IHC
16-0381	Functional Grade* Purified anti-mouse CD38	N/A	N/A	FA FC
13-0381	Biotin anti-mouse CD38	N/A	N/A	FC
11-0381	FITC anti-mouse CD38	488	518	FC
12-0381	PE anti-mouse CD38	488	575	FC
15-0381	PE-Cy5 anti-mouse CD38	488	670	FC
17-0381	APC anti-mouse CD38	633	660	FC
56-0381	Alexa Fluor® 700 anti-mouse CD38 (APC-Cy5.5 Equivalent)	633	723	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 90 monoclonal antibody reacts with the mouse CD38 molecule, an ~42 kDa type II transmembrane protein. CD38 is expressed at increasingly higher levels on B cells at each stage of B-cell differentiation, and is then down-regulated on germinal center B cells and mature plasma cells. Its expression is reported on a subpopulation of thymocytes, mature T cells, and NK cells. Crosslinking of CD38 on the surface of mature, resting B cells induces B-cell proliferation, which is enhanced by co-signals such as IL-4 and LPS. CD38, a counter-receptor for CD31, is an ectoenzyme with cyclase and hydrolase enzymatic activity and is speculated to play a role in lymphocyte activation and differentiation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 90 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This 90 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Oliver, A. M., J. C. Grimaldi, et al. (1999). "Independently ligating CD38 and Fc gammaRIIB relays a dominant negative signal to B cells." Hybridoma 18(2): 113-9.
Oliver, A. M., F. Martin, et al. (1997). "Marginal zone B cells exhibit unique activation, proliferative and immunoglobulin secretory responses." Eur J Immunol 27(9): 2366-74.
Oliver, A. M., F. Martin, et al. (1997). "Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells." J Immunol 158(3): 1108-15.

Related Products

Cat. 11-0389    FITC anti-human CD38 (clone HIT2)
Cat. 12-0389    PE anti-human CD38 (clone HIT2)
Cat. 13-0389    Biotin anti-human CD38 (clone HIT2)
Cat. 14-0389    Affinity Purified anti-human CD38 (clone HIT2)
Cat. 15-0389    PE-Cy5 anti-human CD38 (clone HIT2)
Cat. 17-0389    Allophycocyanin (APC) anti-human CD38 (clone HIT2)
Cat. 25-0389    Phycoerythrin-Cy7 (PE-Cy7) anti-human CD38 (clone HIT2)
Cat. 30-0389    Allophycocyanin-Cy5.5 (APC-Cy5.5) anti-human CD38 (Alexa Fluor® 700 Equivalent) (clone HIT2)
Cat. 56-4321    Alexa Fluor® 700 Rat IgG2a Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.