Product Information

Contents: Alexa Fluor® 700 anti-human CD33
Catalog Number: 56-0338
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: WM-53 (WM53)
Isotype: Mouse IgG1, κ
HLDA No.: VI M505

Staining of normal human peripheral blood cells with Alexa Fluor® 700 Mouse IgG1, K Iso Cntrl (cat. 56-4714) (open histogram) or Alexa Fluor® 700 anti-human CD33 (WM-53) (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0338	Affinity Purified anti-human CD33	N/A	N/A	FC IHC IP MIC
11-0338	FITC anti-human CD33	488	518	FC
12-0338	PE anti-human CD33	488	575	FC
15-0338	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD33	488	670	FC
25-0338	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD33	488	760	FC
56-0338	Alexa Fluor® 700 anti-human CD33 (APC-Cy5.5 Equivalent)	633	723	FC
45-0338	PerCP-Cy5.5 anti-human CD33 (PE-Cy5.5 replacement)	488	690	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This WM-53 (WM53) antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This WM-53 (WM53) antibody has been pre-titrated and tested by flow cytometric analysis of lysed whole blood. Please refer to the antibody vial for the recommended test volume to use for one million cells in a 100 μl total staining volume.

References

Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC. 1987 Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33). Dis Markers. 5(4):215-25.

Related Products

Cat. 56-4714 Alexa Fluor® 700 Mouse IgG1, K Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.