Contents: Alexa FluorŽ 700 anti-human CD33
Catalog Number: 56-0338
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: WM-53 (WM53)
Isotype: Mouse IgG1, κ
HLDA No.: VI M505
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Staining of normal human peripheral blood cells with Alexa FluorŽ 700 Mouse IgG1, K Iso Cntrl (cat. 56-4714) (open histogram) or Alexa FluorŽ 700 anti-human CD33 (WM-53) (colored histogram). Cells in the monocyte gate were used for analysis.
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Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This WM-53 (WM53) antibody has been reported for use in flow cytometric analysis.
The Alexa FluorŽ 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.
Applications Tested
This WM-53 (WM53) antibody has been pre-titrated and tested by flow cytometric analysis of lysed whole blood. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC. 1987 Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33). Dis Markers. 5(4):215-25.
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Cat.
56-4714 Alexa FluorŽ 700 Mouse IgG1, K Isotype Control (APC-Cy5.5 Equivalent)
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Human Cellular Antigens Chart