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Product Information


Contents: Alexa FluorŽ 700 anti-human CD27
Catalog Number: 56-0279
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, gelatin and 0.2% BSA for stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: O323
Isotype: Mouse IgG1, κ
HLDA No.: IV T-186
 
 
data image 1
Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Alexa FluorŽ 700 anti-human CD27 (O323) (colored histogram). Cells in the lymphocyte gate were used for analysis. 

Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
14-0279 Affinity Purified anti-human CD27 N/A N/A FC 
13-0279 Biotin anti-human CD27 N/A N/A FC 
11-0279 FITC anti-human CD27 488 518 FC 
12-0279 PE anti-human CD27 488 575 FC 
15-0279 Phycoerythrin-Cy5 (PE-Cy5) anti-human CD27 488 670 FC 
17-0279 APC anti-human CD27 633 660 FC 
10-0279 **May be discontinued; see superior product APC-Alexa Fluor® 750** APC-Cy7 anti-human CD27 633 760 FC 
25-0279 Phycoerythrin-Cy7 (PE-Cy7) anti-human CD27 488 760 FC 
56-0279 Alexa Fluor® 700 anti-human CD27 (APC-Cy5.5 Equivalent) 633 723 FC 
27-0279 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD27 (APC-Cy7 Equivalent) 633 779 FC 

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description


The O323 monoclonal antibody reacts with human CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by a subset of thymocytes and virtually all mature T cells and is upregulated upon T-cell stimulation. CD27 binds to CD70, and through this interaction, plays an important role in T cell-B cell interaction.


Applications Reported


For research use only, not for diagnostic or therapeutic use. This O323 antibody has been reported for use in flow cytometric analysis.

The Alexa FluorŽ 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.


Applications Tested


This O323 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).


References



Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.


Related Products


Cat. 11-0271    FITC anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 12-0271    PE anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 13-0271    Biotin anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 14-0271    Affinity Purified anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 16-0271    Functional Grade Purified anti-mouse, human, rat CD27 (clone LG.7F9)
Cat. 17-0271    APC anti-mouse, human, rat CD27 (clone LG.7F9)


Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.



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