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Product Information

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Contents: Alexa Fluor® 700 anti-human CD20 Catalog Number: 56-0209 Sizes: 25 tests, 100 tests Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3, gelatin and 0.2% BSA for stabilization Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL. Clone: 2H7 Isotype: Mouse IgG2b, κ HLDA No.: IV B201

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Alexa Fluor® 700 anti-human CD20 (2H7) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0209	Affinity Purified anti-human CD20	N/A	N/A	FC  IH/F
13-0209	Biotin anti-human CD20	N/A	N/A	FC  IH/F
11-0209	FITC anti-human CD20	488	518	FC
12-0209	PE anti-human CD20	488	575	FC
15-0209	PE-Cy5 anti-human CD20	488	670	FC
17-0209	Allophycocyanin (APC) anti-human CD20	633	660	FC
25-0209	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD20	488	760	FC
56-0209	Alexa Fluor® 700 anti-human CD20 (APC-Cy5.5 Equivalent)	633	723	FC
45-0209	PerCP-Cy5.5 anti-human CD20 (PE-Cy5.5 replacement)	488	690	FC
94-0209	eFluorTM 625NC anti-human CD20	350-500	625	FC
95-0209	eFluorTM 650NC anti-human CD20	350-500	650	FC
47-0209	Allophycocyanin-eFluorTM 780 anti-human CD20 (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0209	eFluorTM 450 anti-human CD20	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. This 2H7 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

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Applications Tested

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This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.06 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

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References

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Reinherz, E.L., et al. eds. 1985. Leukocyte Typing II (Vol. I, II, and III). Human Leukocyte Differentiation Antigens detected by Monoclonal Antibodies.
McMichael, A.J., P.C.L. Beverly, et al. eds. 1987. Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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Related Products

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Cat. 56-4732  Alexa Fluor® 700 Mouse IgG2b Isotype Control (APC-Cy5.5 Equivalent)


Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.