human CD20

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Contents: Alexa Fluor® 700 anti-human CD20
Catalog Number: 56-0209
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, gelatin and 0.2% BSA for stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 2H7
Isotype: Mouse IgG2b, κ
HLDA No.: IV B201

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
56-0209-71	25 tests	$150
56-0209-73	100 tests	$295

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Alexa Fluor® 700 anti-human CD20 (2H7) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0209	FITC anti-human CD20	488	518	FC
12-0209	PE anti-human CD20	488	575	FC
13-0209	Biotin anti-human CD20	N/A	N/A	FC IH/F
14-0209	Affinity Purified anti-human CD20	N/A	N/A	FC IH/F
15-0209	PE-Cy5 anti-human CD20	488	670	FC
17-0209	Allophycocyanin (APC) anti-human CD20	633	660	FC
25-0209	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD20	488	760	FC
27-0209	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD20 (APC-Cy7 Equivalent)	633	779	FC
45-0209	PerCP-Cy5.5 anti-human CD20	488	690	FC
56-0209	Alexa Fluor® 700 anti-human CD20 (APC-Cy5.5 Equivalent)	633	723	FC
57-0209	Pacific Blue® anti-human CD20	402	421	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 2H7 monoclonal antibody reacts with human CD20, a 33-36 kDa transmembrane protein. CD20 is expressed by developing B cells as well as mature B cells but not plasma cells. CD20 has been detected at low levels on a small subset of mature T cells. It is suggested that CD20 plays a role in B-cell activation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 2H7 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This 2H7 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.06 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Reinherz, E.L., et al. eds. 1985. Leukocyte Typing II (Vol. I, II, and III). Human Leukocyte Differentiation Antigens detected by Monoclonal Antibodies.
McMichael, A.J., P.C.L. Beverly, et al. eds. 1987. Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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Cat. 56-4732 Alexa Fluor® 700 Mouse IgG2b Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.