Product Information

Contents: Alexa Fluor® 700 anti-mouse CD16/32 - blocks Fc binding
Catalog Number: 56-0161
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 93
Isotype: Rat IgG2a, λ

Staining of BALB/c splenocytes with 0.25 μg of Alexa Fluor® 700 Rat IgG2a Iso Cntrl (cat. 56-4321) (open histogram) or 0.25 μg of Alexa Fluor® 700 anti-mouse CD16/32 (93) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0161	Affinity Purified anti-mouse CD16/32 - blocks Fc binding	N/A	N/A	FA FC
16-0161	Functional Grade* Purified anti-mouse CD16/32 - blocks Fc binding	N/A	N/A	FA FC
13-0161	Biotin anti-mouse CD16/32 - blocks Fc binding	N/A	N/A	FC
11-0161	FITC anti-mouse CD16/32 - blocks Fc binding	488	518	FC
12-0161	PE anti-mouse CD16/32 - blocks Fc binding	488	575	FC
17-0161	APC anti-mouse CD16/32 - blocks Fc binding	633	660	FC
25-0161	PE-Cy7 anti-mouse CD16/32 - blocks Fc binding	488	760	FC
35-0161	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD16/32 - blocks Fc binding	488	690	FC
56-0161	Alexa Fluor® 700 anti-mouse CD16/32 - blocks Fc binding (APC-Cy5.5 Equivalent)	633	723	FC
57-0161	Pacific Blue® anti-mouse CD16/32 - blocks Fc binding	402	421	FC
45-0161	PerCP-Cy5.5 anti-mouse CD16/32 - blocks Fc binding	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fcγ III Receptor) and CD32 (Fcγ II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 93 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This 93 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Oliver, A. M., J. C. Grimaldi, et al. (1999). "Independently ligating CD38 and Fc gammaRIIB relays a dominant negative signal to B cells." Hybridoma 18(2): 113-9.

Related Products

Cat. 56-4321 Alexa Fluor® 700 Rat IgG2a Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.