Product Information

Contents: Alexa Fluor® 700 anti-human CD11c (APC-Cy5.5 Equivalent)
Catalog Number: 56-0116
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 3.9
Isotype: Mouse IgG1, κ
HLDA No.: III NL707

Staining of normal human peripheral blood cells with Alexa Fluor® 700 Mouse IgG1, K Iso Cntrl (cat. 56-4714) (open histogram) or Alexa Fluor® 700 anti-human CD11c (3.9) (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0116	Affinity Purified anti-human CD11c	N/A	N/A	FC IH/F
13-0116	Biotin anti-human CD11c	N/A	N/A	FC
11-0116	FITC anti-human CD11c	488	518	FC
12-0116	PE anti-human CD11c	488	575	FC
17-0116	Allophycocyanin (APC) anti-human CD11c	633	660	FC
25-0116	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD11c	488	760	FC
56-0116	Alexa Fluor® 700 anti-human CD11c (APC-Cy5.5 Equivalent)	633	723	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin α_X chain. CD11c non-covalently associates with β₂ integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 3.9 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This 3.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (1 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 56-0114 Alexa Fluor® 700 anti-mouse CD11c (Integrin aX, p150/90) (APC-Cy5.5 Equivalent) (clone N418)
Cat. 56-4714 Alexa Fluor® 700 Mouse IgG1, K Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.