Product Information

Contents: Alexa Fluor® 700 anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope
Catalog Number: 56-0113
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, gelatin and 0.2% BSA for stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CBRM1/5
Isotype: Mouse IgG1, κ

Staining of 5-minute PMA (50 ng/ml) / ionomycin (1 ug/ml)-stimulated normal human peripheral blood cells. Cells were stained with staining buffer (autofluorescence) (open histogram) or Alexa Fluor® 700 anti-human CD11b (CBRM1/5) (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0113	FITC anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	488	518	FC
12-0113	PE anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	488	575	FC
14-0113	Affinity Purified anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	N/A	N/A	FA FC IP
16-0113	Functional Grade* Purified anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	N/A	N/A	FA FC
51-0113	Alexa Fluor® 647 anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	633	668	FC
56-0113	Alexa Fluor® 700 anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope (APC-Cy5.5 Equivalent)	633	723	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the α chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This CBRM1/5 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This CBRM1/5 antibody has been pre-titrated and tested by flow cytometric analysis of resting and activated human peripheral blood leukocytes. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Oxvig, C., C. Lu, et al. 1999. Conformational changes in tertiary structure near the ligand binding site of an integrin I domain. Proc Natl Acad Sci U S A 96(5): 2215-20.
Weber, C., J. Kitayama, et al. 1996. Differential regulation of beta 1 and beta 2 integrin avidity by chemoattractants in eosinophils. Proc Natl Acad Sci U S A 93(20): 10939-44.
Diamond, M. S. and T. A. Springer 1993. A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen. J Cell Biol 120(2): 545-56.

Related Products

Cat. 56-4714 Alexa Fluor® 700 Mouse IgG1, K Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.