Staining of normal human peripheral blood cells with Anti-Human CD4 FITC (cat. 11-0048) and Mouse IgG2a κ Isotype Control Alexa Fluor® 700 (cat. 56-4724) (left) or Anti-Human CD8a Alexa Fluor® 700 (right). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human CD8a Alexa Fluor® 700 Catalog Number: 56-0086 Concentration: μg size: 0.2 mg/ml; test size: 20 μl (0.06 μg)/test Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: OKT8 (OKT-8) Host/Isotype: Mouse IgG2a
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Cat. No.
Size
Price
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56-0086-71
25 tests
56-0086-73
100 tests
56-0086-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 β chain (CD8 αβ) or as a homodimer (CD8 αα). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Cat. No.14-0088 and clone HIT8a, Cat. No.14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.
The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.
Applications Tested
This OKT8 (OKT-8) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This is offered in 2 formats:
- μg size: This can be used at at 0.06 μg per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 115 to 108 cells/test.
- test size: has been pre-titrated and can be used at test size: 20 μl (0.06 μg)/per test A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. The pre-titrated test size contains 0.2% BSA.
References
Kay HD, Horwitz DA. 1980. Evidence by reactivity with hybridoma antibodies for a probable myeloid origin of peripheral blood cells active in natural cytotoxicity and antibody-dependent cell-mediated cytotoxicity. J Clin Invest. 66(4):847-51.
Sayos J, Wu C, Morra M, Wang N, Zhang X, Allen D, van Schaik S, Notarangelo L, Geha R, Roncarolo MG, Oettgen H, De Vries JE, Aversa G, Terhorst C.The X-linked lymphoproliferative-disease gene product SAP regulates signals induced through the co-receptor SLAM. Nature. 1998 Oct 1;395(6701):462-9. (OKT8, IP, PubMed)
Thomas Y, Sosman J, Irigoyen O, Friedman SM, Kung PC, Goldstein G, Chess L. 1980. Functional analysis of human T cell subsets defined by monoclonal antibodies. I. Collaborative T-T interactions in the immunoregulation of B cell differentiation. J Immunol. 125(6):2402-8.
Campanelli R, Palermo B, Garbelli S, Mantovani S, Lucchi P, Necker A, Lantelme E, Giachino C. 2002. Human CD8 co-receptor is strictly involved in MHC-peptide tetramer-TCR binding and T cell activation. Int Immunol. 14(1):39-44.
