Product Information

Contents: Alexa Fluor® 700 anti-human CD4
Catalog Number: 56-0048
Sizes: 25 tests, 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OKT4 (OKT-4)
Isotype: Mouse IgG2b

Staining of normal human peripheral blood cells with FITC anti-human CD19 (HIB19) (cat. 11-0199) and staining buffer (autofluorescence) (left) or 0.125 μg of Alexa Fluor® 700 anti-human CD4 (OKT4) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0048	Affinity Purified anti-human CD4	N/A	N/A	FC IP
16-0048	Functional Grade* Purified anti-human CD4	N/A	N/A	FC IP
13-0048	Biotin anti-human CD4	N/A	N/A	FC
11-0048	Fluorescein isothiocyanate (FITC) anti-human CD4	488	518	FC
12-0048	Phycoerythrin (PE) anti-human CD4	488	575	FC
17-0048	Allophycocyanin (APC) anti-human CD4	633	660	FC
35-0048	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD4	488	690	FC
51-0048	Alexa Fluor® 647 anti-human CD4	633	668	FC
53-0048	Alexa Fluor® 488 anti-human CD4	488	519	FC
56-0048	Alexa Fluor® 700 anti-human CD4 (APC-Cy5.5 Equivalent)	633	723	FC
57-0048	Pacific Blue® anti-human CD4	402	421	FC
45-0048	PerCP-Cy5.5 anti-human CD4 (PE-Cy5.5 replacement)	488	690	FC
93-0048	eFluor^TM 605^NC anti-human CD4	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This OKT4 (OKT-4) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Reinherz EL, Kung PC, Goldstein G, Schlossman SF. 1979. Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci. 76(8): 4061-5.

Bour S, Boulerice F, Wainberg MA. Inhibition of gp160 and CD4 maturation in U937 cells after both defective and productive infections by human immunodeficiency virus type 1. J Virol. 1991 Dec;65(12):6387-96. (OKT4, IP, PubMed)

Related Products

Cat. 14-0041  Affinity Purified anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 19-0041  Cy5 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 25-0041  PE-Cy7 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 51-0041  Alexa Fluor® 647 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 53-0041  Alexa Fluor® 488 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 12-0042  PE anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 56-0042  Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent) (clone RM4-5)
Cat. 57-0042  Pacific Blue® anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 11-0049  FITC anti-human CD4 (clone RPA-T4)
Cat. 13-0049  Biotin anti-human CD4 (clone RPA-T4)
Cat. 16-0049  Functional Grade Purified anti-human CD4 (clone RPA-T4)
Cat. 17-0049  APC anti-human CD4 (clone RPA-T4)
Cat. 27-0049  Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD4 (APC-Cy7 Equivalent) (clone RPA-T4)
Cat. 56-4732  Alexa Fluor® 700 Mouse IgG2b Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.