human CD4 (L3T4)

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Contents: Alexa Fluor® 700 anti-human CD4
Catalog Number: 56-0048
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OKT4 (OKT-4)
Isotype: Mouse IgG2b

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
56-0048-71	25 tests	$150
56-0048-80	25 ug	$115
56-0048-82	100 ug	$225

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with FITC anti-human CD19 (HIB19) (cat. 11-0199) and staining buffer (autofluorescence) (left) or 0.125 μg of Alexa Fluor® 700 anti-human CD4 (OKT4) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0048	Fluorescein isothiocyanate (FITC) anti-human CD4	488	518	FC
12-0048	Phycoerythrin (PE) anti-human CD4	488	575	FC
13-0048	Biotin anti-human CD4	N/A	N/A	FC
14-0048	Affinity Purified anti-human CD4	N/A	N/A	FC IP
16-0048	Functional Grade* Purified anti-human CD4	N/A	N/A	FC IP
17-0048	Allophycocyanin (APC) anti-human CD4	633	660	FC
35-0048	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD4	488	690	FC
51-0048	Alexa Fluor® 647 anti-human CD4	633	668	FC
53-0048	Alexa Fluor® 488 anti-human CD4	488	519	FC
56-0048	Alexa Fluor® 700 anti-human CD4 (APC-Cy5.5 Equivalent)	633	723	FC
57-0048	Pacific Blue® anti-human CD4	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This OKT4 (OKT-4) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Reinherz EL, Kung PC, Goldstein G, Schlossman SF. 1979. Separation of functional subsets of human T cells by a monoclonal antibody. Proc Natl Acad Sci. 76(8): 4061-5.

Bour S, Boulerice F, Wainberg MA. Inhibition of gp160 and CD4 maturation in U937 cells after both defective and productive infections by human immunodeficiency virus type 1. J Virol. 1991 Dec;65(12):6387-96. (OKT4, IP, PubMed)

Related Products

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Cat. 12-0041    PE anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 13-0041    Biotin anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 14-0041    Affinity Purified anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 15-0041    PE-Cy5 anti-mouse CD4 (L3T4) (clone GK1.5)
Cat. 16-0041    Functional Grade Purified anti-mouse CD4 (L3T4) (clone GK1.5)
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Cat. 19-0041    Cy5 anti-mouse CD4 (L3T4) (clone GK1.5)
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Cat. 56-0041    Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent) (clone GK1.5)
Cat. 10-0042    **May be discontinued; see superior product APC-Alexa Fluor® 750** APC-Cy7 anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 11-0042    FITC anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 12-0042    PE anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 13-0042    Biotin anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 14-0042    Affinity Purified anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 15-0042    PE-Cy5 anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 16-0042    Functional Grade Purified anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 17-0042    APC anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 25-0042    PE-Cy7 anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 27-0042    Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD4 (L3T4) (APC-Cy7 Equivalent) (clone RM4-5)
Cat. 30-0042    Allophycocyanin-Cy5.5 (APC-Cy5.5) anti-mouse CD4 (L3T4) (Alexa Fluor® 700 Equivalent) (clone RM4-5)
Cat. 35-0042    PE-Cy5.5 anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 56-0042    Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent) (clone RM4-5)
Cat. 57-0042    Pacific Blue® anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 11-0043    Fluorescein isothiocyanate (FITC) anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 12-0043    Phycoerythrin (PE) anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 13-0043    Biotin anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 14-0043    Affinity Purified anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 16-0043    Functional Grade Purified anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 10-0049    **May be discontinued; see superior product APC-Alexa Fluor® 750** APC-Cy7 anti-human CD4 (clone RPA-T4)
Cat. 11-0049    FITC anti-human CD4 (clone RPA-T4)
Cat. 12-0049    PE anti-human CD4 (clone RPA-T4)
Cat. 13-0049    Biotin anti-human CD4 (clone RPA-T4)
Cat. 14-0049    Affinity Purified anti-human CD4 (clone RPA-T4)
Cat. 15-0049    PE-Cy5 anti-human CD4 (clone RPA-T4)
Cat. 16-0049    Functional Grade Purified anti-human CD4 (clone RPA-T4)
Cat. 17-0049    APC anti-human CD4 (clone RPA-T4)
Cat. 25-0049    PE-Cy7 anti-human CD4 (clone RPA-T4)
Cat. 27-0049    Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD4 (APC-Cy7 Equivalent) (clone RPA-T4)
Cat. 56-4732    Alexa Fluor® 700 Mouse IgG2b Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.