Product Information

Contents: Alexa Fluor® 700 anti-human CD3 (APC-Cy5.5 Equivalent)
Catalog Number: 56-0038
Sizes: 25 tests, 100 tests, 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: UCHT1
Isotype: Mouse IgG1, κ
HLDA No.: III 471

Staining of normal human peripheral blood cells with FITC anti-human CD19 (HIB19) (cat. 11-0199) and 0.5 μg of Alexa Fluor® 700 Mouse IgG1, K Iso Cntrl (cat. 56-4714) (left) or Alexa Fluor® 700 anti-human CD3 (UCHT1) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0038	Affinity Purified anti-human CD3	N/A	N/A	FA FC IH/F
16-0038	Functional Grade* Purified anti-human CD3	N/A	N/A	FA FC
13-0038	Biotin anti-human CD3	N/A	N/A	FC
11-0038	FITC anti-human CD3	488	518	FC
12-0038	PE anti-human CD3	488	575	FC
15-0038	PE-Cy5 anti-human CD3	488	670	FC
17-0038	APC anti-human CD3	633	660	FC
25-0038	PE-Cy7 anti-human CD3	488	760	FC
56-0038	Alexa Fluor® 700 anti-human CD3 (APC-Cy5.5 Equivalent)	633	723	FC
47-0038	Allophycocyanin-eFluor^TM 780 anti-human CD3 (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0038	eFluor^TM 450 anti-human CD3 (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This UCHT1 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This UCHT1 antibody is offered in 2 formats:

- μg size: has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume. The pre-titrated test size contains BSA and/or gelatin for protein stabilization.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.

Baeten D, De Keyser F, et al. 2004. Tumour necrosis factor alpha independent disease mechanisms in rheumatoid arthritis: a histopathological study on the effect of infliximab on rheumatoid nodules. Ann Rheum Dis. 63(5):489-93. (IHC frozen, PubMed)

Senechal B, Elain G, et al. 2007. Expansion of regulatory T cells in patients with Langerhans cell histiocytosis. PLoS Med. 14;4(8):e253. (IHC frozen, PubMed)

Pollard K, Lunny D, et al. 1987. Fixation, processing, and immunochemical reagent effects on preservation of T-lymphocyte surface membrane antigens in paraffin-embedded tissue. J Histochem Cytochem. 35(11):1329-38. (IHC paraffin and frozen, PubMed)

Related Products

Cat. 56-0032  Alexa Fluor® 700 anti-mouse CD3 (CD3e, epsilon subunit) (APC-Cy5.5 Equivalent) (clone 17A2)
Cat. 56-0037  Alexa Fluor® 700 anti-human CD3 (APC-Cy5.5 Equivalent) (clone OKT3)
Cat. 56-4714  Alexa Fluor® 700 Mouse IgG1, K Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.