human CD3

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Contents: Alexa Fluor® 700 anti-human CD3 (APC-Cy5.5 Equivalent)
Catalog Number: 56-0037
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: OKT3
Isotype: Mouse IgG2a
HLDA No.: VI T6T-R3

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
56-0037-71	25 tests	$150
56-0037-73	100 tests	$295

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with PE anti-human CD19 (HIB19) (cat. 12-0199) and 0.5μg of Alexa Fluor® 700 Mouse IgG2a, K Iso Cntrl (cat. 56-4724) (left) or Alexa Fluor® 700 anti-human CD3 (OKT3) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0037	FITC anti-human CD3	488	518	FC
12-0037	PE anti-human CD3	488	575	FC
14-0037	Affinity Purified anti-human CD3	N/A	N/A	FC
16-0037	Functional Grade* Purified anti-human CD3	N/A	N/A	FA FC
45-0037	PerCP-Cy5.5 anti-human CD3	488	690	FC
51-0037	Alexa Fluor® 647 anti-human CD3	633	668	FC
53-0037	Alexa Fluor® 488 anti-human CD3	488	519	FC
56-0037	Alexa Fluor® 700 anti-human CD3 (APC-Cy5.5 Equivalent)	633	723	FC
57-0037	Pacific Blue® anti-human CD3	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, γ, δ, and ε chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This OKT3 antibody has been reported for use in flow cytometric analysis.

The Alexa Fluor® 700 emits at 723 nm and can be excited with the He-Ne 633 laser. Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochome.

Applications Tested

This OKT3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

Hoffman RA et al. Simple and rapid measurement of human T lymphocytes and their subclasses in peripheral blood. Proc. Natl. Acad. Sci. USA 77: 4914-4917, 1980. PubMed: 81054881
Kung PC and Goldstein G. Complement-fixing monoclonal antibody to human T cells, and methods of preparing same. U.S. Pat. 4,361,549 dated Nov. 30, 1982.
Tanaka Y et al. Productive and lytic infection of human CD4+ type 1 helper T cells with macrophage-tropic human immunodeficiency virus type 1. J. Virol. 71: 465-470, 1997. PubMed: 97138346
Solomon KR et al. The association between glycoslphosphatidylinositol-anchored proteins and hetrotrimeric G protein alpha subunits in lymphocytes. Proc. Natl. Acad. Sci. USA 93: 6053-6058, 1996. PubMed: 96234088
Hutchcroft JE et al. Differential phosphorylation of the T lymphocyte costimulatory receptor CD28. J. Biol. Chem. 271: 13362-13370, 1996. PubMed: 96278754
Adair, JR., et al. 1994. Humanization of the murine anti-human CD3 monoclonal antibody OKT3. Hum Antibodies Hybridomas. 5:41-7.

Related Products

Cat. 56-0032 Alexa Fluor® 700 anti-mouse CD3 (CD3e, epsilon subunit) (APC-Cy5.5 Equivalent) (clone 17A2)
Cat. 56-4724 Alexa Fluor® 700 Mouse IgG2a, K Isotype Control (APC-Cy5.5 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.