Product Information

Contents: Alexa Fluor® 488 anti-mouse CXCR4 (Fusin, CD184)
Catalog Number: 53-9991
Sizes: 25 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 2B11
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 thymocytes with APC anti-mouse CD4 (GK1.5) (cat. 17-0041) and 0.5 μg of Alexa Fluor® 488 Rat IgG2b Isotype Control (cat. 53-4031) (left) or 0.5 μg of Alexa Fluor® 488 anti-mouse CXCR4 (2B11) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9991	Affinity Purified anti-mouse CXCR4 (Fusin, CD184)	N/A	N/A	FC IH/F WB
16-9991	Functional Grade* Purified anti-mouse CXCR4 (Fusin, CD184)	N/A	N/A	FA FC
12-9991	Phycoerythrin (PE) anti-mouse CXCR4 (Fusin, CD184)	488	575	FC
51-9991	Alexa Fluor® 647 anti-mouse CXCR4 (Fusin, CD184)	633	668	FC
53-9991	Alexa Fluor® 488 anti-mouse CXCR4 (Fusin, CD184)	488	519	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 2B11 monoclonal antibody reacts with mouse and human CXCR4, also known as CD184, Fusin, LESTR, or HUMSTR. Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. In addition it has been shown to play a role in lymphocyte migration. The ligand for CXCR4 is SDF1. CXCR4 is required for maintenance of granulocyte progenitors in the bone marrow and essential for B cell development. Expression of CXCR4 is different between mice and human. In the thymus CXCR4 is restricted to CD4+CD8+ cells while in the spleen predominant expression is found on B lymphocytes.
The monoclonal antibody 2B11 recognizes the amino terminus of CXCR4 and has been reported to have functional activity; it does not block SDF1a but causes internalization of the receptor.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 2B11 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 2B11 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Forster R, Kremmer E, Schubel A, Breitfeld D, Kleinschmidt A, Nerl C, Bernhardt G, Lipp M. Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation. J Immunol. 1998 Feb 1;160(3):1522-31. (2B11, FC, WB, FA, PubMed)

Schabath R, Muller G, Schubel A, Kremmer E, Lipp M, Forster R. The murine chemokine receptor CXCR4 is tightly regulated during T cell development and activation. J Leukoc Biol. 1999 Dec;66(6):996-1004.(2B11, FC, WB PubMed)

Stumm RK, Rummel J, Junker V, Culmsee C, Pfeiffer M, Krieglstein J, Hollt V, Schulz S. A dual role for the SDF-1/CXCR4 chemokine receptor system in adult brain: isoform-selective regulation of SDF-1 expression modulates CXCR4-dependent neuronal plasticity and cerebral leukocyte recruitment after focal ischemia. J Neurosci. 2002 Jul 15;22(14):5865-78. (2B11, WB, PubMed)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.