Anti-Glial Fibrillary Acid Protein (GFAP) Alexa FluorŽ 488
RUO: For Research Use Only
Immunocytochemistry of fixed and permeabilized C6 cells using 10 μg/ml of Mouse IgG1 Isotype Control Alexa FluorŽ 488 (cat. 53-4714)(left) or Anti-GFAP Alexa FluorŽ 488 (right). Nuclei were counterstained with DAPI.
Contents: Anti-Glial Fibrillary Acid Protein (GFAP) Alexa FluorŽ 488 Catalog Number: 53-9892 Concentration: 0.5 mg/mL Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light-sensitive material. Clone: GA5 (GA-5, G-A-5) Host/Isotype: Mouse IgG1
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53-9892-80
25 ug
53-9892-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa FluorŽ 647 conjugated products with eFluorŽ 660 conjugated products.
Description
This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, α-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This GA5 antibody has been reported for use in immunohistology staining of frozen tissue sections and immunocytochemistry.
Applications Tested
This GA5 antibody has been tested by immunocytochemistry of fixed and permeabilized C6 cells. This can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Quinlan RA, Brenner M, Goldman JE, Messing A. GFAP and its role in Alexander disease. Exp Cell Res. 2007 Jun 10;313(10):2077-87.
McLendon RE, Bigner DD. Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations. Brain Pathol. 1994 Jul;4(3):221-8.
Mokuno K, Kamholz J, Behrman T, Black C, Sessa M, Feinstein D, Lee V, Pleasure D. Neuronal modulation of Schwann cell glial fibrillary acidic protein (GFAP). J Neurosci Res. 1989 Aug;23(4):396-405. (GA5, WB)
Rasmussen S, Bock E, Warecka K, Althage G. Quantitation of glial fibrillary acidic protein in human brain tumours. Br J Cancer. 1980 Jan;41(1):113-6.
Legal
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
