Product Information

Contents: Alexa Fluor® 488 anti-mouse Toll-like receptor 4 (CD284, TLR4, TLR-4)
Catalog Number: 53-9041
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: UT41
Isotype: Mouse IgG1

Staining of C57Bl/6 peritoneal-exudate cells with 0.5 μg of Alexa Fluor® 488 Mouse IgG1, K Iso Cntrl (cat. 53-4714) (open histogram) or 0.5 μg of Alexa Fluor® 488 anti-mouse TLR4 (UT41) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9041	Affinity Purified anti-mouse Toll-like receptor 4 (CD284, TLR4, TLR-4)	N/A	N/A	FC
13-9041	Biotin anti-mouse Toll-like receptor 4 (CD284, TLR4, TLR-4)	N/A	N/A	FC
12-9041	Phycoerythrin (PE) anti-mouse Toll-like receptor 4 (CD284, TLR4, TLR-4)	488	575	FC
53-9041	Alexa Fluor® 488 anti-mouse Toll-like receptor 4 (CD284, TLR4, TLR-4)	488	519	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The UT41 monoclonal antibody was generated against BaF3 cells overexpressing mouse TLR4. So far, at least twelve members of the Toll family have been identified. This family of type I transmembrane protein is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4, which is approximately 100 kDa, is expressed by myeloid lineage cells.

The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex . Please contact eBioscience Technical Support for further information.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This UT41 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This UT41 antibody has been tested by flow cytometric analysis of mouse peritoneal exudate cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nomura, F., S. Akashi, et al. 2000. Cutting edge: endotoxin tolerance in mouse peritoneal macrophages correlates with down-regulation of surface toll-like receptor 4 expression. J Immunol 164(7): 3476-9.

Sato, S., F. Nomura, et al. 2000. Synergy and cross-tolerance between toll-like receptor (TLR) 2- and TLR4-mediated signaling pathways. J Immunol 165(12): 7096-101.

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Cat. 11-8317  Fluorescein isothiocyanate (FITC) mouse anti-human IFN-alpha2 (clone 225.C)
Cat. 12-8317  Phycoerythrin (PE) mouse anti-human IFN-alpha2 (clone 225.C)
Cat. 17-8317  Allophycocyanin (APC) mouse anti-human IFN-alpha2 (clone 225.C)
Cat. 13-9924  Biotin anti-mouse Toll-like receptor 4/ MD-2 (TLR4/ MD-2, TLR-4/ MD2) (clone MTS510)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.