Product Information

Contents: Alexa Fluor® 488 anti-SSEA-4 (stage-specific embryonic antigen-4)
Catalog Number: 53-8843
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioMC-813-70 (MC-813-70)
Isotype: Mouse IgG3

Staining of 2102Ep cell line with FITC Mouse IgG3 Iso Cntrl (cat. 11-4742) (open histogram) or Alexa Fluor® 488 anti-human SSEA-4 (MC-813-70) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8843	Affinity Purified anti-SSEA-4 (stage-specific embryonic antigen-4)	N/A	N/A	FC IHC
13-8843	Biotin anti-SSEA-4 (stage-specific embryonic antigen-4)	N/A	N/A	FC
12-8843	Phycoerythrin (PE) anti-SSEA-4 (stage-specific embryonic antigen-4)	488	575	FC
53-8843	Alexa Fluor® 488 anti-SSEA-4 (stage-specific embryonic antigen-4)	488	519	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioMC-813-70 monoclonal antibody reacts with a glycolipid carbohydrate epitope on Stage-specific embryonic antigen-4 (SSEA-4) protein. Expression is found on human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) with a decrease in expression upon differentiation. Recently expression has also been found on bone marrow mesenchymal stem cells.

The eBioMC-813-70 antibody has been reported to crossreact to mouse, rabbit, canine, chicken, and Old and New World monkeys.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioMC-813-70 (MC-813-70) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioMC-813-70 (MC-813-70) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 20 μl (1.0 μg) per million cells in a 100 μl total staining volume.

References

Williams SA, Stanley P. Complex N-glycans or core 1-derived O-glycans are not required for the expression of stage-specific antigens SSEA-1, SSEA-3, SSEA-4, or Le(Y) in the preimplantation mouse embryo. Glycoconj J. 2009 Apr;26(3):335-47. (mouse reactivity)

Müller T, Fleischmann G, Eildermann K, Mätz-Rensing K, Horn PA, Sasaki E, Behr R A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics. Hum Reprod. 2009 Feb 27. (marmoset reactvity)

Shiue YL, Tailiu JJ, Liou JF, Lu HT, Tai C, Shiau JW, Chen LR. Establishment of the long-term in vitro culture system for chicken primordial germ cells. Reprod Domest Anim. 2009 Feb;44(1):55-61 (chicken reactivity)

Wilcox JT, Semple E, Gartley C, Brisson BA, Perrault SD, Villagómez DA, Tayade C, Becker S, Lanza R, Betts DH. Characterization of canine embryonic stem cell lines derived from different niche microenvironments. Stem Cells Dev. 2009 Jan 9. (canine reactivity)

Müller T, Eildermann K, Dhir R, Schlatt S, Behr R. Glycan stem-cell markers are specifically expressed by spermatogonia in the adult non-human primate testis. Hum Reprod. 2008 Oct;23(10):2292-8 (Callithrix jacchus, Macaca mulatta and Macaca silenus crossreactivity)

Gang EJ, Bosnakovski D, Figueiredo CA, Visser JW, Perlingeiro RC. SSEA-4 identifies mesenchymal stem cells from bone marrow. Blood. 2007 Feb 15;109(4):1743-51.

Kannagi R, Cochran NA, Ishigami F, Hakomori S, Andrews PW, Knowles BB, Solter D. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J. 1983;2(12):2355-61.

Krupnick JG, Damjanov I, Damjanov A, Zhu ZM, Fenderson BA. Globo-series carbohydrate antigens are expressed in different forms on human and murine teratocarcinoma-derived cells. Int J Cancer. 1994 Dec 1;59(5):692-8.

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