Product Information

Contents: Alexa Fluor® 488 anti-mouse/human SSEA-3 (stage-specific embryonic antigen-3)
Catalog Number: 53-8833
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioMC-631 (MC-631)
Isotype: Rat IgM

Staining of the 2102Ep cell line with Alexa Fluor® 488 Rat IgM Iso Cntrl (cat. 53-4341) (open histogram) or Alexa Fluor® 488 anti-human SSEA-3 (MC-631) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8833	Affinity Purified anti-human/mouse SSEA-3 (stage-specific embryonic antigen-3)	N/A	N/A	FC IHC
13-8833	Biotin anti-human/mouse SSEA-3 (stage-specific embryonic antigen-3)	N/A	N/A	FC
12-8833	Phycoerythrin (PE) anti-human/mouse SSEA-3 (stage-specific embryonic antigen-3)	488	575	FC
53-8833	Alexa Fluor® 488 anti-human/mouse SSEA-3 (stage-specific embryonic antigen-3)	488	519	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioMC-631 (MC-631) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 20 μl (1.0 μg) per million cells in a 100 μl total staining volume.

References

Andrews PW, Goodfellow PN, Shevinsky LH, Bronson DL, Knowles BB. Cell-surface antigens of a clonal human embryonal carcinoma cell line: morphological and antigenic differentiation in culture. Int J Cancer. 1982 May 15;29(5):523-31.

Kannagi R, Cochran NA, Ishigami F, Hakomori S, Andrews PW, Knowles BB, Solter D. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells. EMBO J. 1983;2(12):2355-61.

Related Products

Cat. 53-4341 Alexa Fluor® 488 Rat IgM Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.