Contents: Alexa FluorŽ 488 anti-mouse/human SSEA-3 (stage-specific embryonic antigen-3)
Catalog Number: 53-8833
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioMC-631 (MC-631)
Isotype: Rat IgM
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Staining of the 2102Ep cell line with Alexa FluorŽ 488 Rat IgM Iso Cntrl (cat. 53-4341) (open histogram) or Alexa FluorŽ 488 anti-human SSEA-3 (MC-631) (colored histogram). Total viable cells were used for analysis.
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Description
The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioMC-631 (MC-631) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 20 μl (1.0 μg) per million cells in a 100 μl total staining volume.
References
Andrews PW, Goodfellow PN, Shevinsky LH, Bronson DL, Knowles BB. Cell-surface antigens of a clonal human embryonal carcinoma cell line: morphological and antigenic differentiation in culture.
Int J Cancer. 1982 May 15;29(5):523-31.
Kannagi R, Cochran NA, Ishigami F, Hakomori S, Andrews PW, Knowles BB, Solter D. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells.
EMBO J. 1983;2(12):2355-61.
Related Products
Cat.
53-4341 Alexa FluorŽ 488 Rat IgM Isotype Control
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Mouse Cellular Antigens Chart
Human Cellular Antigens Chart