Product Information

Contents: Alexa Fluor® 488 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)
Catalog Number: 53-7349
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, gelatin and 0.2% BSA for stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MAb11
Isotype: Mouse IgG1, κ

Staining of 5 hour PMA and Ionomycin-stimulated (in the presence of brefeldinA) normal human peripheral blood mononuclear cells with APC anti-human CD4 (OKT4) (cat. 17-0048) and Alexa Fluor® 488 Mouse IgG1, K Iso Cntrl (cat. 53-4714) (left) or Alexa Fluor® 488 anti-human TNFa (MAb11) (right). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7349	Affinity Purified anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	IC Flow
13-7349	Biotin anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	N/A	N/A	ELISA det ELISPOT det
11-7349	FITC anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	518	IC Flow
12-7349	PE anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	575	IC Flow
17-7349	APC anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	660	IC Flow
25-7349	Phycoerythrin-Cy7 (PE-Cy7) anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	760	IC Flow
51-7349	Alexa Fluor® 647 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	633	668	IC Flow
53-7349	Alexa Fluor® 488 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	519	IC Flow
57-7349	Pacific Blue® anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	402	421	IC Flow
45-7349	PerCP-Cy5.5 anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a)	488	690	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MAb11 antibody reacts with human tumor necrosis factor-alpha (TNF-α ), a 17 kDa cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4⁺ T cells. TNF-α has cytolytic activity against a range of tumor cells and is important in immune regulation. TNF-α forms dimers and trimers and also exists as a 26 kDa membrane bound form. The MAb11 antibody is a neutralizing antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MAb11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This MAb11 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood mononuclear cells. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Danis, V. A., G. M. Franic, et al. (1991). "Effects of granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-2, interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha) and IL-6 on the production of immunoreactive IL-1 and TNF-alpha by human monocytes." Clin Exp Immunol 85(1): 143-50.
Rathjen, D. A., K. Cowan, et al. (1991). "Antigenic structure of human tumour necrosis factor: recognition of distinct regions of TNF alpha by different tumour cell receptors." Mol Immunol 28(1-2): 79-86.

Related Products

Cat. 53-4714    Alexa Fluor® 488 Mouse IgG1, K Isotype Control
Cat. 88-7347    Human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
Cat. 14-7348    Affinity Purified anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone MAb1)
Cat. 16-7348    Functional Grade Purified anti-human TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone MAb1)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.