Product Information

Contents: Alexa Fluor® 488 anti-mouse IFNg (Interferon-gamma, IFN-g)
Catalog Number: 53-7311
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: XMG1.2
Isotype: Rat IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
53-7311-80	25 ug	$115
53-7311-82	100 ug	$225

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7311	FITC anti-mouse IFNg (Interferon-gamma, IFN-g)	488	518	IC Flow
12-7311	PE anti-mouse IFNg (Interferon-gamma, IFN-g)	488	575	IC Flow
13-7311	Biotin anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA
14-7311	Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA cap
16-7311	Functional Grade* Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	NU
17-7311	APC anti-mouse IFNg (Interferon-gamma, IFN-g)	633	660	IC Flow
19-7311	Cy5 anti-mouse IFNg (Interferon-gamma, IFN-g)	633	670	IC Flow
25-7311	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IFNg (Interferon-gamma, IFN-g)	488	760	IC Flow
45-7311	PerCP-Cy5.5 anti-mouse IFNg (Interferon-gamma, IFN-g)	488	690	IC Flow
51-7311	Alexa Fluor® 647 anti-mouse IFNg (Interferon-gamma, IFN-g)	633	668	IC Flow
53-7311	Alexa Fluor® 488 anti-mouse IFNg (Interferon-gamma, IFN-g)	488	519	IC Flow
57-7311	Pacific Blue® anti-mouse IFNg (Interferon-gamma, IFN-g)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The XMG1.2 antibody reacts with mouse interferon-γ, a 15-20 kDa cytokine secreted by Th1 cells.

Usage

For research use only, not for diagnostic or therapeutic use. This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Related Products

Cat. 11-7311    FITC anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2)
Cat. 12-7311    PE anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2)
Cat. 13-7311    Biotin anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2)
Cat. 14-7311    Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2)
Cat. 16-7311    Functional Grade Purified anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2)
Cat. 17-7311    APC anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2)
Cat. 19-7311    Cy5 anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2)
Cat. 25-7311    Phycoerythrin-Cy7 (PE-Cy7) anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2)
Cat. 51-7311    Alexa Fluor® 647 anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2)
Cat. 57-7311    Pacific Blue® anti-mouse IFNg (Interferon-gamma, IFN-g) (clone XMG1.2)
Cat. 13-7312    Biotin anti-mouse IFNg (Interferon-gamma, IFN-g) (clone R4-6A2)
Cat. 14-7312    Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g) (clone R4-6A2)
Cat. 16-7312    Functional Grade Purified anti-mouse IFNg (Interferon-gamma, IFN-g) (clone R4-6A2)
Cat. 36-7312    In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade Biotin anti-mouse IFNg (Interferon-gamma, IFN-g) (clone R4-6A2)
Cat. 14-7313    Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g) (clone AN-18)
Cat. 16-7313    Functional Grade Purified anti-mouse IFNg (Interferon-gamma, IFN-g) (clone AN-18)
Cat. 88-7914    Mouse IFNg (Interferon-gamma, IFN-g) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)

References

Abrams, J. S., M. G. Roncarolo, et al. (1992). "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." Immunol Rev 127: 5-24.

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.