Product Information

Contents: Alexa Fluor® 488 anti-human IL-17A (Interleukin-17A, IL17A)
Catalog Number: 53-7179
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio64DEC17
Isotype: Mouse IgG1, κ

Human PBMC's were stimulated with PMA/Ionomycin overnight in the presence of monensin. Cells were harvested and surface stained with APC anti-human CD3 (clone UCHT1, cat.17-0038), then fixed, permeabilized, and stained with AlexaFluor 488 conjugated mouse IgG1 isotype control (cat. 53-4714), left, or eBio64DEC17 antibody, right.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7179	Affinity Purified anti-human IL-17A (Interleukin-17A)	N/A	N/A	ELISA IHC(Paraffin)
13-7179	Biotin anti-human IL-17A (Interleukin-17A, IL17A)	N/A	N/A	ELISA det ELISPOT det
11-7179	Fluorescein isothiocyanate (FITC) anti-human IL-17A (Interleukin-17A)	488	518	IC Flow
12-7179	Phycoerythrin (PE) anti-human IL-17A (Interleukin-17A, IL17A)	488	575	IC Flow
51-7179	Alexa Fluor® 647 anti-human IL-17A (Interleukin-17A, IL17A)	633	668	IC Flow
53-7179	Alexa Fluor® 488 anti-human IL-17A (Interleukin-17A, IL17A)	488	519	IC Flow
45-7179	PerCP-Cy5.5 anti-human IL-17A (Interleukin-17A)	488	690	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio64DEC17 antibody reacts with human IL-17A. The eBio64DEC17 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Intracellular staining by eBio64DEC17 antibody identifies the same cell population as the eBio64CAP17 antibody, as can be seen in co-staining experiments using both antibodies. Click here for link to data 51-7179.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The eBio64DEC17 antibody has been reported for use as the detection antibody in human IL-17A ELISA and ELISPOT assays, as well as for neutralization and intracellular staining.

Applications Tested

This eBio64DEC17 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.

Related Products

Cat. 53-4714    Alexa Fluor® 488 Mouse IgG1, K Isotype Control
Cat. 53-7172    Alexa Fluor® 488 anti-mouse IL-17A (Interleukin-17A, IL17A) (clone eBioTC11-18H10.1)
Cat. 16-7178    Functional Grade Purified anti-human IL-17A (Interleukin-17A, IL17A) (clone eBio64CAP17)
Cat. 88-7876    Human IL-17A (Interleukin-17A, IL17A) ELISPOT Ready-Set-Go!
Cat. 14-8179    Recombinant Human IL-17A (Interleukin-17A, IL17A)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.