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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe eBio17B7 antibody reacts with mouse IL-17A. The eBio17B7 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor. IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-γ and IL-4, have each been found to negatively regulate the generation of these Th-17 cells. Applications ReportedFor research use only, not for diagnostic or therapeutic use. The eBio17B7 antibody has been reported useful for neutralization of mouse IL-17A, for ELISA/ELISPOT detection, and for intracellular staining for flow cytometric analysis. Applications TestedThe fluorochrome-conjugated eBio17B7 antibody has been tested by intracellular staining for flow cytometric analysis of PMA and Ionomycin-restimulated splenocytes cultured under Th17-polarizing conditions for 3 days. It can be used at less than or equal to 0.50 µg/million cells/100 ul staining volume. Staining has been successfully done using the Foxp3 buffer system (cat 00-5523). Please contact Technical Support Department at tech@ebioscience.com. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Related ProductsCat. 00-4222 eBioscience Flow Cytometry Staining Buffer Cat. 53-4321 Alexa Fluor® 488 Rat IgG2a Isotype Control Cat. 53-7179 Alexa Fluor® 488 anti-human IL-17A (Interleukin-17A, IL17A) (clone eBio64DEC17) Cat. 88-7234 Mouse IL-23 (p19/p40, IL23) ELISA Ready-SET-Go! Cat. 88-7370 Mouse IL-17A (Interleukin-17A, IL17A) ELISPOT Ready-Set-Go! Cat. 88-7374 Mouse IL-17A (Interleukin-17A, IL17A) Tissue Culture ELISA Ready-SET-Go! Cat. 00-8222 eBioscience IC Fixation Buffer Cat. 34-8231 Carrier-Free Recombinant Mouse IL-23 (Interleukin-23, IL23) Cat. 00-8333 eBioscience Permeabilization Buffer(10X) Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc. |
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