Contents: Alexa Fluor® 488 anti-human IL-12 (Interleukin-12) p40/70
Catalog Number: 53-7129
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: C8.6
Isotype: Mouse IgG1, κ
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Intracellular staining of unstimulated (left) and 2 hour IFNg then LPS overnight activated (right) normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Alexa Fluor® 488 anti-human IL-12 p40/70 (C8.6) (colored histogram). Cells in the monocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-7129 |
Affinity Purified anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 |
N/A |
N/A |
IC Flow
NU
WB
|
| 16-7129 |
Functional Grade* Purified anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 |
N/A |
N/A |
NU
|
| 13-7129 |
Biotin anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 |
N/A |
N/A |
ELISA det
|
| 12-7129 |
PE anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 |
488 |
575 |
IC Flow
|
| 51-7129 |
Alexa Fluor® 647 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 |
633 |
668 |
IC Flow
|
| 53-7129 |
Alexa Fluor® 488 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 |
488 |
519 |
IC Flow
|
| 48-7129 |
eFluorTM 450 anti-human IL-12 (Interleukin-12) / IL-23 (Interleukin-23) p40/70 (Pacific Blue® replacement) |
405 |
450 |
IC Flow
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.
Applications Reported
For research use only, not for diagnostic or therapeutic use.
Applications Tested
This C8.6 antibody has been pre-titrated and tested by intracellulkar staining of IFNγ LPS actiavted human PBMCs. This can be used at 20 μl (0.03 μg) per million cells in a 100 μl total staining volume.
References
Cho, D., et al. 1996. Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 172: 29-34.
Aragane, Y, et al. 1994. IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 153: 5366-72.
Related Products
Cat. 53-4714 Alexa Fluor® 488 Mouse IgG1, K Isotype Control
Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
