mouse IL-2 (Interleukin-2)

Contents: Alexa Fluor® 488 anti-mouse IL-2 (Interleukin-2, IL2)
Catalog Number: 53-7021
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: JES6-5H4
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
53-7021-80	25 ug	$115
53-7021-82	100 ug	$225

*International customers: Please contact your distributor for region specific pricing.

IL-2 versus CD4: Mouse splenocytes were stimulated with ConA for 2 days, followed by IL-2 and IL-4 for 3 days, and restimulated with immobilized anti-CD3 and soluble anti-CD28 in the presence of Brefeldin A for 5 hours. The cells were surface stained with APC anti-mouse CD4 alone (left) or in conjunction with Alexa fluor 488 anti-mouse IL-2 (right).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7021	FITC anti-mouse IL-2 (Interleukin-2, IL2)	488	518	IC Flow
12-7021	PE anti-mouse IL-2 (Interleukin-2, IL2)	488	575	IC Flow
13-7021	Biotin anti-mouse IL-2 (Interleukin-2, IL2)	N/A	N/A	ELISA det ELISPOT det IP
14-7021	Affinity Purified anti-mouse IL-2 (Interleukin-2, IL2)	N/A	N/A	IC Flow IP
16-7021	Functional Grade* Purified anti-mouse IL-2 (Interleukin-2, IL2)	N/A	N/A	IC Flow
17-7021	APC anti-mouse IL-2 (Interleukin-2, IL2)	633	660	IC Flow
36-7021	In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade* Biotin anti-mouse IL-2 (Interleukin-2, IL2)	N/A	N/A	IVCCA
45-7021	PerCP-Cy5.5 anti-mouse IL-2 (Interleukin-2)	488	690	IC Flow
51-7021	Alexa Fluor® 647 anti-mouse IL-2 (Interleukin-2, IL2)	633	668	IC Flow
53-7021	Alexa Fluor® 488 anti-mouse IL-2 (Interleukin-2, IL2)	488	519	IC Flow
57-7021	Pacific Blue® anti-mouse IL-2 (Interleukin-2, IL2)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The JES6-5H4 antibody has been reported useful for ELISA, ELISPOT, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested

The Alexa Fluor® 488 JES6-5H4 antibody has been tested by intracellular staining and flow cytometric analysis and can be used at less than or equal to 0.25 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Abrams, J. S., M. G. Roncarolo, et al. (1992). Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 127: 5-24.
Sander, B., I. Hoiden, et al. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Meth. 166(2): 201-14.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

Related Products

Cat. 53-4331    Alexa Fluor® 488 Rat IgG2b Isotype Control
Cat. 14-7022    Affinity Purified anti-mouse IL-2 (Interleukin-2, IL2) (clone JES6-1A12)
Cat. 16-7022    Functional Grade Purified anti-mouse IL-2 (Interleukin-2, IL2) (clone JES6-1A12)
Cat. 88-7024    Mouse IL-2 (Interleukin-2, IL2) ELISA Ready-SET-Go!
Cat. 88-7924    Mouse IL-2 (Interleukin-2, IL2) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.