Contents: Alexa FluorŽ 488 anti-human AITR / GITR
Catalog Number: 53-5875
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioAITR
Isotype: Mouse IgG1
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Human PBMCs were cultured for 3 days with PHA, stained with PE anti-human ICOS (cat 12-9948), and either Alexa Fluor 488 mouseIgG1 isotype control (cat 53-4714) (left) or Alexa Fluor 488 eBioAITR (right). Total viable cells were used for analysis.
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Description
The monoclonal antibody reacts with human AITR, Activation Inducible TNFR family member, an approximately 25 kDa member of the TNFR superfamily. AITR mRNA is detected in lymph node, peripheral blood leukocytes and weakly in spleen. AITR mRNA expression was upregulated within 24 hour on PMA/ionomycin or PHA stimulated PBMC. At the protein level, AITR is expressed by a small population of activated PBMC. AITR associates with TRAF1, TRAF2 and TRAF3 and induces nuclear factor NF-κB activation via TRAF2. Recently TL6 (AITRL) has been reported as the ligand for AITR. Interaction of AITR with AITRL is important for cross-talk between T lymphocytes and endothelial cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioAITR antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioAITR antibody has been pre-titrated and tested by flow cytometric analysis of PHA-stimulated normal peripheral blood mononuclear cells. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Kwon B, Yu KY, Ni J, Yu GL, Jang IK, Kim YJ, Xing L, Liu D, Wang SX, Kwon BS. 1999. J Biol Chem. 274(10):6056-61. Identification of a novel activation-inducible protein of the tumor necrosis factor receptor superfamily and its ligand.
Related Products
Cat.
53-4714 Alexa FluorŽ 488 Mouse IgG1, K Isotype Control
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Human Cellular Antigens Chart