Product Information

Contents: Alexa Fluor® 488 anti-human MICA/MICB (MIC A/B)
Catalog Number: 53-5788
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, gelatin and 0.2% BSA for stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 6D4
Isotype: Mouse IgG2a, κ

Staining of HeLa cells with Alexa Fluor® 488 Mouse IgG2a, K Iso Cntrl (cat. 53-4724) (open histogram) or Alexa Fluor® 488 anti-human MICA/MICB (6D4) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5788	Affinity Purified anti-human MICA/MICB (MIC A/B)	N/A	N/A	FA FC IHC IP
16-5788	Functional Grade* Purified anti-human MICA/MICB (MIC A/B)	N/A	N/A	FA FC IHC IP
12-5788	Phycoerythrin (PE) anti-human MICA/MICB (MIC A/B)	488	575	FC
53-5788	Alexa Fluor® 488 anti-human MICA/MICB (MIC A/B)	488	519	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 6D4 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I chain-related (MIC), MICA and MICB proteins. MICA and MICB are related proteins of 83% amino acid similarity, and show homology with classical human leukocyte antigen (HLA) molecules. The structure of MICA and MICB are similar to classical HLA class I chains, however they do not bind β2 microglobulin or bind peptide typical of HLA class I. MICA and MICB are expressed on the cell surface of endothelial cells, fibroblasts, gastric epithelium and PHA-stimulated T cells, and act as a ligand for NKGD2 expressed on the surface of NK cells, γδ T cells and αβ CD8+ T cells. There is evidence to suggest that human cytomegalovirus (HCMV) subverts NK cell detection by inhibiting the function of MICB. Furthermore, MICA and MICB expression has been detected in several epithelial tumours isolated from breast, lung, ovary, prostate, colon and kidney.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 6D4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 6D4 antibody has been pre-titrated and tested by flow cytometric analysis of HeLa cells. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Groh V, Steinle A, Bauer S, Spies T. Recognition of stress-induced MHC molecules by intestinal epithelial gammadelta T cells. Science. 1998 Mar 13;279(5357):1737-40. (6D4, FA, FC, PubMed)

Li Z, Groh V, Strong RK, Spies T. A single amino acid substitution causes loss of expression of a MICA allele. Immunogenetics. 2000 Mar;51(3):246-8. (6D4, FC, WB, PubMed)

Jinushi M, Takehara T, Kanto T, Tatsumi T, Groh V, Spies T, Miyagi T, Suzuki T, Sasaki Y, Hayashi N. Critical role of MHC class I-related chain A and B expression on IFN-alpha-stimulated dendritic cells in NK cell activation: impairment in chronic hepatitis C virus infection. J Immunol. 2003 Feb 1;170(3):1249-56. (6D4, FC, FA, PubMed)

Edelmann W, Zervas M, et al. 1996. Neuronal abnormalities in microtubule-associated protein 1B mutant mice. Proc Natl Acad Sci U S A. 93(3):1270-5. (IHC frozen, PubMed)

Hankey KG, Drachenberg CB, et al. 2002. MIC expression in renal and pancreatic allografts. Transplantation. 73(2):304-6. (IHC paraffin, PubMed)

Groh V, Rhinehart R, et al. 1999. Broad tumor-associated expression and recognition by tumor-derived gamma delta T cells of MICA and MICB. Proc Natl Acad Sci U S A. 96(12):6879-84. (IHC frozen and paraffin, PubMed)

Related Products

Cat. 53-4724 Alexa Fluor® 488 Mouse IgG2a, K Isotype Control
Cat. 13-5878 Biotin anti-human NKG2D / CD314 (activating) (clone 1D11)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.