Contents: Alexa FluorŽ 488 anti-mouse Podoplanin (T1a, gp38, stromal cell marker)
Catalog Number: 53-5381
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio8.1.1 (8.1.1)
Isotype: Golden Syrian Hamster IgG
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Staining of TE-71 cells with 0.25 μg of Alexa FluorŽ 488 Golden Syrian Hamster IgG Iso Cntrl (cat. 53-4914) (open histogram) or 0.25 μg of Alexa FluorŽ 488 anti-mouse Podoplanin (8.1.1) (colored histogram). Total viable cells were used for analysis.
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Description
The 8.1.1 monoclonal antibody reacts with mouse podoplanin (T1a, gp38, aggrus), a 43 kDa transmembrane glycoprotein, named for its expression kidney glomerular epithelial cells (podocytes). In addition, Podoplanin is expressed in epithelial and mesothelial cells such as intestinal epithelium, alveolar type I cells, podocytes, and mesothelium of the visceral peritoneum. It was also shown to be a potent marker for lymphatic endothelium. Podoplanin is also expressed by subcapsular epithelial cells of the murine thymus. Mice deficient in Podoplanin die at birth because of a respiratory defect and congenital lymphedema due to a failure in lymphatic pattern formation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio8.1.1 (8.1.1) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio8.1.1 (8.1.1) antibody has been tested by flow cytometric analysis of TE-71 cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Farr A, Nelson A, Hosier S. Characterization of an antigenic determinant preferentially expressed by type I epithelial cells in the murine thymus.
J Histochem Cytochem. 1992 May;40(5):651-64. (
8.1.1, mAb development,
PubMed)
Farr AG, Berry ML, Kim A, Nelson AJ, Welch MP, Aruffo A.
Characterization and cloning of a novel glycoprotein expressed by stromal cells in T-dependent areas of peripheral lymphoid tissues.
J Exp Med. 1992 Nov 1;176(5):1477-82. (
8.1.1, IHC,
PubMed)
Mahtab EA, Wijffels MC, Van Den Akker NM, Hahurij ND, Lie-Venema H, Wisse LJ, Deruiter MC, Uhrin P, Zaujec J, Binder BR, Schalij MJ, Poelmann RE, Gittenberger-De Groot AC. Cardiac malformations and myocardial abnormalities in podoplanin knockout mouse embryos: Correlation with abnormal epicardial development.
Dev Dyn. 2008 Mar;237(3):847-57.
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Cat.
53-4914 Alexa FluorŽ 488 Golden Syrian Hamster IgG Isotype Control (clone n/a)
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Mouse Cellular Antigens Chart