Product Information

Contents: Alexa Fluor® 488 anti-mouse F4/80 Antigen - Pan Macrophage Marker, BM8
Catalog Number: 53-4801
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BM8
Isotype: Rat IgG2a, κ

Staining of BALB/c splenocytes with 0.25 μg of Alexa 488 Rat IgG2a Iso Cntrl (open histogram) or 0.25 μg of Alexa Fluor® 488 anti-mouse F4/80 (BM8) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-4801	Affinity Purified anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8)	N/A	N/A	FC IH/F IHC(Paraffin)
16-4801	Functional Grade* Purified anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8)	N/A	N/A	FC
13-4801	Biotin anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8)	N/A	N/A	FC
11-4801	FITC anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8)	488	518	FC
12-4801	PE anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8)	488	575	FC
15-4801	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8)	488	670	FC
17-4801	Allophycocyanin (APC) anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8)	633	660	FC
25-4801	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8)	488	760	FC
51-4801	Alexa Fluor® 647 anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8)	633	668	FC
53-4801	Alexa Fluor® 488 anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8)	488	519	FC
57-4801	Pacific Blue® anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8)	402	421	FC
27-4801	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8) (APC-Cy7 Equivalent)	633	779	FC
45-4801	PerCP-Cy5.5 anti-mouse F4/80 Antigen (Pan Macrophage Marker, BM8)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 125 kDa transmembrane protein. The F4/80 antigen is expressed by a majority of mature macrophages and is the best marker for this population of cells. However, other cell types such as Langerhans cells and liver Kupffer cells have been reported to express this antigen. Expression of F4/80 commences during early myeloid development with approximately 30% of BM cells staining with BM8 and is upregulated on all BM cells stimulated in vitro with M-CSF. It has been shown that some cytokines downregulate the expression of F4/80 resulting in lack of F4/80 antigen on a subpopulation of macrophages, especially in the lymphoid microenvironment in vivo.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This BM8 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This BM8 antibody has been tested by flow cytometric analysis of mouse spleen and bone marrow cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Schaller E, Macfarlane AJ, Rupec RA, Gordon S, McKnight AJ, Pfeffer K. 2002. Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol Cell Biol. 22(22):8035-43.

Leenen PJ, de Bruijn MF, Voerman JS, Campbell PA, van Ewijk W. 1994. Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods. 174(1-2):5-19.

Zwadlo G, Brocker EB, von Bassewitz DB, Feige U, Sorg C. 1985. A monoclonal antibody to a differentiation antigen present on mature human macrophages and absent from monocytes. J Immunol. 134(3):1487-92.

Murayama, T., M. Yokode, et al. 1999. Intraperitoneal administration of anti-c-fms monoclonal antibody prevents initial events of atherogenesis but does not reduce the size of advanced lesions in apolipoprotein E-deficient mice. Circulation. 99(13):1740-6. (IHC frozen, PubMed)

Geutskens, S., T. Otonkoski, et al. 2005. Macrophages in the murine pancreas and their involvement in fetal endocrine development in vitro. J Leukoc Biol. 78(4): 845-52. (IHC frozen, PubMed)

Torzewski, M., P. Shaw, et al. 2004. Reduced in vivo aortic uptake of radiolabeled oxidation-specific antibodies reflects changes in plaque composition consistent with plaque stabilization. Aterioscler Thromb Vasc Biol. 24(12): 2307-12. (IHC paraffin, PubMed)

Mackler, A., L.M. Green, et al. 2000. Distribution and activation of uterine mononuclear phagocytes in peripartum endometrium and myometrium of the mouse. Biol Reprod. 62(5):1193-200. (IHC paraffin, PubMed)

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Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.