Product Information

Contents: Alexa Fluor® 488 anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)
Catalog Number: 53-3172
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio927
Isotype: Rat IgG2b

Staining of SJL splenocytes with APC anti-mouse CD11c (N418) (cat. 17-0114) and 0.5 μg of Alexa Fluor® 488 Rat IgG2b Isotype Control (cat. 53-4031) (left) or 0.5 μg of Alexa Fluor® 488 Mouse PDCA-1 (pDC Ag-1, BST2, CD317) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-3172	Affinity Purified anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	N/A	N/A	FC
16-3172	Functional Grade* Purified anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	N/A	N/A	FA FC
13-3172	Biotin anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	N/A	N/A	FC
11-3172	Fluorescein isothiocyanate (FITC) anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	488	518	FC
12-3172	Phycoerythrin (PE) anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	488	575	FC
51-3172	Alexa Fluor® 647 anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	633	668	FC
53-3172	Alexa Fluor® 488 anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC)	488	519	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naïve mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8.

The eBio927 monoclonal antibody has also been shown to have functional activity. The epitope recognized by eBio927 is distinct from eBio129c; thus, the antibodies can be used to costain, purify and identify pDCs.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio927 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio927 antibody has been tested by flow cytometric analysis of SJL mouse splenocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Blasius A.L., E. Giurisat, M. Cella, R.D. Schreiber, A.S. Shaw, M. Colonna. 2006. Bone Marrow Stromal Cell Antigen 2 Is a Specific Marker of Type I IFN-Producing Cells in the Naïve Mouse, but a Promiscuous Cell Surface Antigen Following IFN Stimulation. J Immunol. 177:3260-3265 (927, FC, FA)

Related Products

Cat. 53-4031 Alexa Fluor® 488 Rat IgG2b Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.