Product Information

Contents: Alexa Fluor® 488 anti-human CD144 (VE-Cadherin)
Catalog Number: 53-1449
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 16B1
Isotype: Mouse IgG1

Staining of HUVEC cells with 0.25 μg of Alexa Fluor® 488 Mouse IgG1, K Iso Cntrl (cat. 53-4714) (open histogram) or 0.25 μg of Alexa Fluor® 488 anti-human CD144 (VE-Cadherin) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1449	Affinity Purified anti-human CD144 (VE-Cadherin)	N/A	N/A	FC WB
13-1449	Biotin anti-human CD144 (VE-Cadherin)	N/A	N/A	FC
12-1449	Phycoerythrin (PE) anti-human CD144 (VE-Cadherin)	488	575	FC
17-1449	Allophycocyanin (APC) anti-human CD144 (VE-Cadherin)	633	660	FC
53-1449	Alexa Fluor® 488 anti-human CD144 (VE-Cadherin)	488	519	FC
56-1449	Alexa Fluor® 700 anti-human CD144 (VE-Cadherin)	633	723	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 16B1 antibody reacts with human CD144, also known as VE-cadherin and cadherin-5. The cadherin family of receptors, which are calcium-dependent adhesion molecules, is known to be involved in homophilic cell interactions. VE-cadherin, which is 140 kDa, is localized at the intercellular boundaries of endothelial cells in blood and lymphatic vessels in several tissues. It is thought to play a role in vascular permeability and remodeling.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 16B1 antibody has been reported for use in flow cytometric analysis, and immunocytochemistry.

Applications Tested

This 16B1 antibody has been pre-titrated and tested by flow cytometric analysis of Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at 20 μl per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells. Stem Cells. 2007 Feb;25(2):490-9. (16B1, Immunocytofluorescence, PubMed)

Suzuki S, Sano K, and Tanihara H. 1991. Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue. Cell Regul 2: 261-270.

Breviario F, Caveda L, Corada M, Martin-Padura I, Navarro P, Golay J, Introna M, Gulino D, Lampugnani MG, and Dejana E. 1995. Functional properties of human vascular endothelial cadherin (7B4/Cadherin-5), an endothelium-specific cadherin. Arterioscler Thromb Vasc Biol 15: 1229-1239.

Vincent PA, Xiao K, Buckley KM, and Kowalczyk AP. 2004. VE-Cadherin: adhesion at arm's length. Am J Physiol Cell Physiol 286: 987-997.

Related Products

Cat. 53-4714 Alexa Fluor® 488 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.