mouse CD144 (VE-Cadherin)

Contents: Alexa Fluor® 488 anti-mouse CD144 (VE-Cadherin)
Catalog Number: 53-1441
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioBV13 (BV13)
Isotype: Rat IgG1

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
53-1441-80	25 ug	$195
53-1441-82	100 ug	$365

*International customers: Please contact your distributor for region specific pricing.

Staining of bEnd.3 cells with 0.5 μg of Alexa Fluor® 488 Rat IgG1 Iso Cntrl (cat. 53-4301) (open histogram) or 0.5 μg of Alexa Fluor® 488 anti-mouse CD144 (eBioBV13) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1441	Affinity Purified anti-mouse CD144 (VE-Cadherin)	N/A	N/A	FA FC IH/F IP WB
16-1441	Functional Grade* Purified anti-mouse CD144 (VE-Cadherin)	N/A	N/A	FA FC IH/F IP WB
51-1441	Alexa Fluor® 647 anti-mouse CD144 (VE-Cadherin)	633	668	FC
53-1441	Alexa Fluor® 488 anti-mouse CD144 (VE-Cadherin)	488	519	FC IH/F
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The BV13 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioBV13 (BV13) antibody has been reported for use in flow cytometric analysis, and immunohistology staining of frozen tissue sections.

Applications Tested

This eBioBV13 (BV13) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Corada M, Mariotti M, Thurston G, Smith K, Kunkel R, Brockhaus M, Lampugnani MG, Martin-Padura I, Stoppacciaro A, Ruco L, McDonald DM, Ward PA, Dejana E. Vascular endothelial-cadherin is an important determinant of microvascular integrity in vivo. Proc Natl Acad Sci U S A. 1999 Aug 17;96(17):9815-20. (BV13, FA, IHC, PubMed)

Liao F, Li Y, O'Connor W, Zanetta L, Bassi R, Santiago A, Overholser J, Hooper A, Mignatti P, Dejana E, Hicklin DJ, Bohlen P. Monoclonal antibody to vascular endothelial-cadherin is a potent inhibitor of angiogenesis, tumor growth, and metastasis. Cancer Res. 2000 Dec 15;60(24):6805-10. (BV13, FA, PubMed)

Crosby CV, Fleming PA, Argraves WS, Corada M, Zanetta L, Dejana E, Drake CJ. VE-cadherin is not required for the formation of nascent blood vessels but acts to prevent their disassembly. Blood. 2005 Apr 1;105(7):2771-6. Epub 2004 Dec 16. (BV13, FA, PubMed)

Related Products

Cat. 53-1449 Alexa Fluor® 488 anti-human CD144 (VE-Cadherin) (clone 16B1)
Cat. 53-4301 Alexa Fluor® 488 Rat IgG1 Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.