Product Information

Contents: Alexa Fluor® 488 anti-mouse CD107b (LAMP-2)
Catalog Number: 53-1072
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioABL-93 (ABL-93)
Isotype: Rat IgG2a, κ

Intracellular staining of thioglycolate-induced peritoneal-exudate cells with 0.5 μg of Alexa Fluor® 488 Rat IgG2a Iso Cntrl (cat. 53-4321) (open histogram) or Alexa Fluor® 488 anti-mouse CD107b (ABL-93) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1072	Affinity Purified anti-mouse CD107b (LAMP-2)	N/A	N/A	IC Flow IHC IP
13-1072	Biotin anti-mouse CD107b (LAMP-2)	N/A	N/A	IC Flow
11-1072	Fluorescein isothiocyanate (FITC) anti-mouse CD107b (LAMP-2)	488	518	IC Flow
51-1072	Alexa Fluor® 647 anti-mouse CD107b (LAMP-2)	633	668	IC Flow
53-1072	Alexa Fluor® 488 anti-mouse CD107b (LAMP-2)	488	519	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioABL-93 monoclonal antibody reacts with mouse CD107b, also known as LAMP-2 (lysosomal-associated membrane protein 2). CD107b, an approximately 120 kDa protein, is highly glycosylated and expressed intracellularly on myeloid cells and epithelial cells. It is reported that CD107b is identical to the mouse Mac-3 antigen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioABL-93 (ABL-93) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This eBioABL-93 (ABL-93) antibody has been tested by intracellular staining and flow cytometric analysis of thioglycollate elicited peritoneal macrophages. This can be used at less than or equal to 1.0 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Chen JW, Murphy TL, Willingham MC, Pastan I, August JT. Identification of two lysosomal membrane glycoproteins. J Cell Biol. 1985 Jul;101(1):85-95. (ABL-93, IP, IHC, PubMed)

Cha Y, Holland SM, August JT. The cDNA sequence of mouse LAMP-2. Evidence for two classes of lysosomal membrane glycoproteins. J Biol Chem. 1990 Mar 25;265(9):5008-13. (ABL-93, affinity-chromatography, PubMed)

Chen JW, Chen GL, D'Souza MP, Murphy TL, August JT. Lysosomal membrane glycoproteins: properties of LAMP-1 and LAMP-2. Biochem Soc Symp. 1986;51:97-112.

Related Products

Cat. 53-1078 Alexa Fluor® 488 anti-human CD107b (LAMP-2) (clone eBioH4B4 (H4B4))
Cat. 53-4321 Alexa Fluor® 488 Rat IgG2a Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.