mouse CD107a (CD107-a, LAMP-1, LAMP1)

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Contents: Alexa Fluor® 488 anti-mouse CD107a (LAMP-1)
Catalog Number: 53-1071
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio1D4B (1D4B)
Isotype: Rat IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
53-1071-80	25 ug	$85
53-1071-82	100 ug	$195

*International customers: Please contact your distributor for region specific pricing.

Intracellular staining of thioglycolate-induced peritoneal-exudate cells with 0.25 μg of Alexa Fluor® 488 Rat IgG2a Iso Cntrl (cat. 53-4321) (open histogram) or 0.25 μg of Alexa Fluor® 488 anti-mouse CD107a (1D4B) (colored histogram). Total cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-1071	Affinity Purified anti-mouse CD107a (LAMP-1)	N/A	N/A	IC Flow IH/F IP WB
13-1071	Biotin anti-mouse CD107a (LAMP-1)	N/A	N/A	IC Flow
12-1071	Phycoerythrin (PE) anti-mouse CD107a (LAMP-1)	488	575	IC Flow
51-1071	Alexa Fluor® 647 anti-mouse CD107a (LAMP-1)	633	668	IC Flow
53-1071	Alexa Fluor® 488 anti-mouse CD107a (LAMP-1)	488	519	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio1D4B monoclonal antibody reacts with mouse CD107a, also known as lysosome-associated membrane protein-1 (LAMP-1). CD107a is a type I, integral lysosomal membrane protein expressed constitutively at high levels by all cells. The majority of CD107a is localised to the limiting membrane of late endosomes-lysosomes, and LAMP-1 and LAMP-2 contribute approximately 50% of all lysosomal membrane protein. CD107a consists of an extensively glycosylated lumenal domain, a transmembrane domain and a short cytoplasmic tail. LAMP-1 deficient mice are viable and fertile, however mice deficient in both LAMP-1 and LAMP-2 have an embryonic lethal phenotype. LAMP-1 has been implicated in a variety of cellular functions, including cancer metastasis, and is a marker for lysosomal storage disorders.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio1D4B (1D4B) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This eBio1D4B (1D4B) antibody has been tested by intracellular staining and flow cytometric analysis of thioglycolate-induced peritoneal exudate cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Rohrer J, Schweizer A, Russell D, Kornfeld S. The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane. J Cell Biol. 1996 Feb;132(4):565-76. (1D4B, immunoelectron microscopy, PubMed)

Gough NR, Zweifel ME, Martinez-Augustin O, Aguilar RC, Bonifacino JS, Fambrough DM. Utilization of the indirect lysosome targeting pathway by lysosome-associated membrane proteins (LAMPs) is influenced largely by the C-terminal residue of their GYXXphi targeting signals. J Cell Sci. 1999 Dec;112 ( Pt 23):4257-69. (1D4B, IF, PubMed)

Chen JW, Pan W, D'Souza MP, August JT. Lysosome-associated membrane proteins: characterization of LAMP-1 of macrophage P388 and mouse embryo 3T3 cultured cells. Arch Biochem Biophys. 1985 Jun;239(2):574-86. (1D4B, CD107a purification, PubMed)

Related Products

Cat. 53-1079 Alexa Fluor® 488 anti-human CD107a (LAMP-1) (clone eBioH4A3)
Cat. 53-4321 Alexa Fluor® 488 Rat IgG2a Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.