Contents: Alexa FluorŽ 488 anti-human CD100 (SEMA4D)
Catalog Number: 53-1009
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio133-1C6 (133-1C6)
Isotype: Mouse IgM
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Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Alexa FluorŽ 488 anti-human CD100 (133-1C6) (colored histogram). Cells in the lymphocyte gate were used for analysis.
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Description
The eBio133-1C6 monoclonal antibody reacts with human CD100, a member of the class IV semaphorin family also called sema4D. CD100 promotes angiogenesis, invasive growth, proliferation, and anti-apoptosis of cancer cells in vitro. Furthermore CD100 is a costimulatory molecule in adaptive immune responses by switching off negative signalling from CD72. In addition to CD72 another ligand is Plexin-B1. It has been shown that higher expression levels of CD100 are correlated with poor survival in soft tissue sarcoma patients.
Expression is found in both hematopoietic and non-hematopoietic cells.CD100 is expressed on resting T cells and NK cells and somewhat weakly on B cells, but activation upregulates expression on all cell types especially T cells. CD100 can also exist as a soluble form.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio133-1C6 (133-1C6) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio133-1C6 (133-1C6) antibody has been pre-titrated and tested by flow cytometric of human peripheral blood cells. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.
References
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Human Cellular Antigens Chart