Product Information

Contents: Alexa Fluor® 488 anti-human CD56 (N-CAM, NCAM)
Catalog Number: 53-0568
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CB56
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with Alexa Fluor® 488 Mouse IgG1, K Iso Cntrl (cat. 53-4714) (blue histogram) or Alexa Fluor® 488 anti-human CD56 (CB56) (purple histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0568	Affinity Purified anti-human CD56 (N-CAM, NCAM)	N/A	N/A	FC
13-0568	Biotin anti-human CD56 (N-CAM, NCAM)	N/A	N/A	FC
53-0568	Alexa Fluor® 488 anti-human CD56 (N-CAM, NCAM)	488	519	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The CB56 monoclonal antibody reacts with human CD56, also known as Neural cell Adhesion Molecule (NCAM). CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells. In the hematopoietic system, CD56 is expressed on NK cells and NKT cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This CB56 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This CB56 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 5 μl (1 μg) per million cells in a 100 μl total staining volume.

References

Hayakawa Y, Huntington ND, Nutt SL, Smyth MJ. Functional subsets of mouse natural killer cells. Immunol Rev. 2006 Dec;214:47-55.

Kishimoto, T., A.E.G., von dem Borne, et al. eds. 1998. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing, Inc. London.

Related Products

Cat. 53-4714 Alexa Fluor® 488 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.