human Integrin Alpha 5

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Contents: Alexa Fluor® 488 anti-human Integrin Alpha 5 (fibronectin receptor α, VLA-5α, CD49e)
Catalog Number: 53-0496
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioSAM-1 (SAM-1, SAM1)
Isotype: Mouse IgG1

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
53-0496-71	25 tests	$150
53-0496-73	100 tests	$295

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with APC anti-human CD3 (UCHT1) (cat. 17-0038) and Alexa Fluor® 488 Mouse IgG1, K Iso Cntrl (cat. 53-4714) (left) or Alexa Fluor® 488 anti-human Integrin Alpha 5 (eBioSam-1) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0496	Affinity Purified anti-human CD49e (Integrin alpha 5)	N/A	N/A	FC IHC IP
13-0496	Biotin anti-human CD49e (Integrin alpha 5)	N/A	N/A	FC
53-0496	Alexa Fluor® 488 anti-human CD49e (Integrin alpha 5)	488	519	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioSAM-1 monoclonal antibody reacts with human integrin alpha 5, also known as fibronectin receptor alpha chain, very late activation antigen 5 alpha, and CD49e. Integrins are composed of an alpha chain and a beta chain, which non-covalently associate to form the functional integrin. Integrin heterodimers participate in cell surface-mediated signaling and adhesion functions. Integrin alpha 5 undergoes post-translational cleavage in its extracellular domain to yield disulfide linked light and heavy chains that join with Integrin beta 1 (CD29) to form the fibronectin receptor, also known as the very late activation antigen-5 (VLA-5) complex. Integrin alpha 5 is expressed on thymocytes, T cells, monocytes, platelets, early B cells, and activated B cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioSAM-1 (SAM-1, SAM1) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioSAM-1 (SAM-1, SAM1) antibody has been pre-titrated and tested by flow cytometric analysis of human PBMCs. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Xiao J, Messinger Y, Jin J, Myers DE, Bolen JB, Uckun FM. Signal transduction through the beta1 integrin family surface adhesion molecules VLA-4 and VLA-5 of human B-cell precursors activates CD19 receptor-associated protein-tyrosine kinases. J Biol Chem. 1996 Mar 29;271(13):7659-64. (SAM-1, FC, PubMed)

Grassi F, Dezutter-Dambuyant C, McIlroy D, Jacquet C, Yoneda K, Imamura S, Boumsell L, Schmitt D, Autran B, Debre P, Hosmalin A. Monocyte-derived dendritic cells have a phenotype comparable to that of dermal dendritic cells and display ultrastructural granules distinct from Birbeck granules. J Leukoc Biol. 1998 Oct;64(4):484-93. (SAM-1, FC, IHC, PubMed)

Santoni G, Birarelli P, Hong LJ, Gamero A, Djeu JY, Piccoli M. An alpha 5 beta 1-like integrin receptor mediates the binding of less pathogenic Candida species to fibronectin. J Med Microbiol. 1995 Nov;43(5):360-7. (SAM-1, FC, PubMed)

Theodore PR, Simon AR, Warrens AN, Sackstein R, Sykes M. Porcine mononuclear cells adhere to human fibronectin independently of very late antigen-5: implications for donor-specific tolerance induction in xenotransplantation. Xenotransplantation. 2002 Jul;9(4):277-89. (SAM-1, FA, PubMed)

Related Products

Cat. 53-4714 Alexa Fluor® 488 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.