Contents: Alexa Fluor® 488 anti-mouse/human CD45R (B220)
Catalog Number: 53-0452
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RA3-6B2
Isotype: Rat IgG2a, κ
HLDA No.: N/A
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Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 μg of Alexa Fluor® 488 anti-mo/hu B220 (RA3-6B2) (colored histogram). Cells in the lymphocyte gate were used for analysis.
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Description
The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the
lpr/lpr mutant mouse also express this antigen.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This RA3-6B2 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Morse, H. C. d., W. F. Davidson, et al. (1982). "Abnormalities induced by the mutant gene Ipr: expansion of a unique lymphocyte subset."
J Immunol 129(6): 2612-5.
Coffman, R. L. (1982). "Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development."
Immunol Rev 69: 5-23.
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53-4321 Alexa Fluor® 488 Rat IgG2a Isotype Control
Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Mouse Cellular Antigens Chart
Human Cellular Antigens Chart