Product Information

Contents: Alexa Fluor® 488 anti-human CD25 (Interleukin-2 Receptor, IL-2R)
Catalog Number: 53-0259
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BC96
Isotype: Mouse IgG1, κ
HLDA No.: V T-072

Staining of normal human peripheral blood cells with Alexa Fluor® 647 anti-human CD4 (OKT4) (cat. 51-0048) and Alexa Fluor® 488 Mouse IgG1, K Iso Cntrl (cat. 53-4714) (left) or Alexa Fluor® 647 anti-human CD25 (Interleukin-2 Receptor, IL-2R) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0259	May be discontinued; see superior product Alexa Fluor® 488 FITC anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	518	FC
12-0259	PE anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	575	FC
13-0259	Biotin anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	N/A	N/A	FC
14-0259	Affinity Purified anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	N/A	N/A	FC
15-0259	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	670	FC
17-0259	Allophycocyanin (APC) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	633	660	FC
25-0259	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	760	FC
45-0259	PerCP-Cy5.5 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	690	FC
53-0259	Alexa Fluor® 488 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	519	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This BC96 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of human PBMC. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

When staining for CD25 (with emission at 518nm) it is recommended to use Alexa Fluor 488 conjugated antibodies, rather than FITC for optimal detection.

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 53-4714 Alexa Fluor® 488 Mouse IgG1, K Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.