Contents: Alexa FluorŽ 488 anti-human CD25 (Interleukin-2 Receptor, IL-2R)
Catalog Number: 53-0259
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BC96
Isotype: Mouse IgG1, κ
HLDA No.: V T-072
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Price |
Qty |
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*International customers: Please contact your distributor for region specific pricing.
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Staining of normal human peripheral blood cells with Alexa FluorŽ 647 anti-human CD4 (OKT4) (cat. 51-0048) and Alexa FluorŽ 488 Mouse IgG1, K Iso Cntrl (cat. 53-4714) (left) or Alexa FluorŽ 647 anti-human CD25 (Interleukin-2 Receptor, IL-2R) (right). Cells in the lymphocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-0259 |
**May be discontinued; see superior product Alexa Fluor® 488** FITC anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
518 |
FC
|
| 12-0259 |
PE anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
575 |
FC
|
| 13-0259 |
Biotin anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
N/A |
N/A |
FC
|
| 14-0259 |
Affinity Purified anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
N/A |
N/A |
FC
|
| 15-0259 |
Phycoerythrin-Cy5 (PE-Cy5) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
670 |
FC
|
| 17-0259 |
Allophycocyanin (APC) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
633 |
660 |
FC
|
| 25-0259 |
Phycoerythrin-Cy7 (PE-Cy7) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
760 |
FC
|
| 45-0259 |
PerCP-Cy5.5 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
690 |
FC
|
| 53-0259 |
Alexa Fluor® 488 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
519 |
FC
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This BC96 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of human PBMC. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.
When staining for CD25 (with emission at 518nm) it is recommended to use Alexa Fluor 488 conjugated antibodies, rather than FITC for optimal detection.
References
Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Related Products
Cat.
53-4714 Alexa FluorŽ 488 Mouse IgG1, K Isotype Control
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Human Cellular Antigens Chart