Product Information

Contents: Alexa Fluor® 488 anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra)
Catalog Number: 53-0253
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio3C7 (3C7)
Isotype: Rat IgG2b, κ

Staining of BALB/c splenocytes with PE anti-mouse CD4 (RM4-5) (cat. 12-0042) and 0.06 μg of Alexa Fluor® 488 Rat IgG2b Iso Cntrl (cat. 53-4331) (left) or 0.06 μg of Alexa Fluor® 488 anti-mouse CD25 (3C7) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
53-0253	Alexa Fluor® 488 anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra)	488	519	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio3C7 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor a chain (IL-2Ra). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of the IL-2 receptor is inhibited by the 3C7 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation. The epitopes recognized by eBio3C7, PC61.5 and eBio7D4 are all different allowing for depletion with one antibody (typically PC61.5) and subsequent detection with another CD25 antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio3C7 (3C7) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio3C7 (3C7) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Klug DB, Carter C, Crouch E, Roop D, Conti CJ, Richie ER. Interdependence of cortical thymic epithelial cell differentiation and T-lineage commitment. Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11822-7. (eBio3C7, IH/F, PubMed)

Ortega G, Robb RJ, Shevach EM, Malek TR. The murine IL 2 receptor. I. Monoclonal antibodies that define distinct functional epitopes on activated T cells and react with activated B cells. J Immunol. 1984 Oct;133(4):1970-5.

Related Products

Cat. 53-0251    Alexa Fluor® 488 anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) (clone PC61.5)
Cat. 53-0252    Alexa Fluor® 488 anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) (clone eBio7D4 (7D4))
Cat. 53-0259    Alexa Fluor® 488 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) (clone BC96)
Cat. 53-4031    Alexa Fluor® 488 Rat IgG2b Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.