Product Information

Contents: Alexa Fluor® 488 anti-mouse CD11c (Integrin aX, p150/90)
Catalog Number: 53-0114
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: N418
Isotype: Armenian Hamster IgG

Staining of C57Bl/6 splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and staining buffer (autofluorescence) (left) or 0.125 μg of Alexa Fluor® 488 anti-mouse CD11c (N418) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0114	Affinity Purified anti-mouse CD11c (Integrin aX, p150/90)	N/A	N/A	FC IH/F IP
16-0114	Functional Grade* Purified anti-mouse CD11c (Integrin aX, p150/90)	N/A	N/A	FC
13-0114	Biotin anti-mouse CD11c (Integrin aX, p150/90)	N/A	N/A	FC
11-0114	FITC anti-mouse CD11c (Integrin aX, p150/90)	488	518	FC
12-0114	PE anti-mouse CD11c (Integrin aX, p150/90)	488	575	FC
15-0114	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD11c (Integrin aX, p150/90)	488	670	FC
17-0114	APC anti-mouse CD11c (Integrin aX, p150/90)	633	660	FC
25-0114	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD11c (Integrin aX, p150/90)	488	760	FC
35-0114	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD11c (Integrin aX, p150/90)	488	690	FC
51-0114	Alexa Fluor® 647 anti-mouse CD11c (Integrin aX, p150/90)	633	668	FC
53-0114	Alexa Fluor® 488 anti-mouse CD11c (Integrin aX, p150/90)	488	519	FC
56-0114	Alexa Fluor® 700 anti-mouse CD11c (Integrin aX, p150/90) (APC-Cy5.5 Equivalent)	633	723	FC
45-0114	PerCP-Cy5.5 anti-mouse CD11c (Integrin aX, p150/90) (PE-Cy5.5 replacement)	488	690	FC
94-0114	eFluor^TM 625^NC anti-mouse CD11c (Integrin aX, p150/90)	350-500	625	FC
47-0114	Allophycocyanin-eFluor^TM 780 anti-mouse CD11c (Integrin aX, p150/90) (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0114	eFluor^TM 450 anti-mouse CD11c (Integrin aX, p150/90) (Pacific Blue® Replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The N418 monoclonal antibody reacts with mouse CD11c, the integrin α_X. CD11c non-covalently associates with β₂ integrin to form the CD11c/CD18 heterodimer. CD11c is expressed by dendritic cells, a subset of Intestinal Intraepithelial Lymphocytes (IEL) and some activated T cells. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. N418 binds to CD11c on splenic dendritic cells in the T-dependent areas of mouse spleen and precipitates a 150, 90 kDa heterodimer.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This N418 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This N418 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Xin KQ, Mizukami H, Urabe M, Toda Y, Shinoda K, Yoshida A, Oomura K, Kojima Y, Ichino M, Klinman D, Ozawa K, Okuda K. Induction of robust immune responses against human immunodeficiency virus is supported by the inherent tropism of adeno-associated virus type 5 for dendritic cells. J Virol. 2006 Dec;80(24):11899-910. (N418, FC, PubMed)

Ohteki T, Tada H, Ishida K, Sato T, Maki C, Yamada T, Hamuro J, Koyasu S. Essential roles of DC-derived IL-15 as a mediator of inflammatory responses in vivo. J Exp Med. 2006 Oct 2;203(10):2329-38. (N418, IHC frozen, PubMed)

Zhang J, Kawashima N, Suda H, Nakano Y, Takano Y, Azuma M. The existence of CD11c+ sentinel and F4/80+ interstitial dendritic cells in dental pulp and their dynamics and functional properties. Int Immunol. 2006 Sep;18(9):1375-84. (N418, IHC frozen, PubMed)

Guiducci C, Vicari AP, Sangaletti S, Trinchieri G, Colombo MP. Redirecting in vivo elicited tumor infiltrating macrophages and dendritic cells towards tumor rejection. Cancer Res. 2005 Apr 15;65(8):3437-46. (N418, IHC frozen, PubMed)

Esche C, Gambotto A, Satoh Y, Gerein V, Robbins PD, Watkins SC, Lotze MT, Shurin MR. CD154 inhibits tumor-induced apoptosis in dendritic cells and tumor growth.Eur J Immunol. 1999 Jul;29(7):2148-55.

Finkelman FD, Lees A, Birnbaum R, Gause WC, Morris SC. Dendritic cells can present antigen in vivo in a tolerogenic or immunogenic fashion.J Immunol. 1996 Aug 15;157(4):1406-14.

Crowley MT, Inaba K, Witmer-Pack MD, Gezelter S, Steinman RM. Use of the fluorescence activated cell sorter to enrich dendritic cells from mouse spleen. J Immunol Methods. 1990 Oct 4;133(1):55-66.

Metlay JP, Witmer-Pack MD, Agger R, Crowley MT, Lawless D, Steinman RM. The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J Exp Med. 1990 May 1;171(5):1753-71.

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Cat. 53-4888 Alexa Fluor® 488 Armenian Hamster IgG Isotype Control (clone eBio299Arm)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.