Product Information

Contents: Alexa Fluor® 488 anti-mouse CD8 (beta subunit; CD8b, Ly-3)
Catalog Number: 53-0083
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioH35-17.2 (H35-17.2)
Isotype: Rat IgG2b, κ

Staining of BALB/c splenocytes with APC anti-mouse CD8a (53-6.7) (cat. 17-0081) and 0.125 μg of Alexa Fluor® 488 Rat IgG2b Iso Cntrl (cat. 53-4331) (left) or 0.125 μg of Alexa Fluor® 488 anti-mouse CD8 (beta subunit; CD8b, Ly-3) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0083	Affinity Purified anti-mouse CD8 (beta subunit; CD8b, Ly-3)	N/A	N/A	FC IH/F IHC(Paraffin) IP
13-0083	Biotin anti-mouse CD8 (beta subunit; CD8b, Ly-3)	N/A	N/A	FC
11-0083	Fluorescein isothiocyanate (FITC) anti-mouse CD8 (beta subunit; CD8b, Ly-3)	488	518	FC
12-0083	Phycoerythrin (PE) anti-mouse CD8 (beta subunit; CD8b, Ly-3)	488	575	FC
15-0083	Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD8 (beta subunit; CD8b, Ly-3)	488	670	FC
17-0083	Allophycocyanin (APC) anti-mouse CD8 (beta subunit; CD8b, Ly-3)	633	660	FC
53-0083	Alexa Fluor® 488 anti-mouse CD8 (beta subunit; CD8b, Ly-3)	488	519	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8β molecule. The CD8β chain associates with the CD8α chain to form the CD8α/β heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic αβ TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioH35-17.2 (H35-17.2) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Lefrancois L. Phenotypic complexity of intraepithelial lymphocytes of the small intestine. J Immunol. 1991 Sep 15;147(6):1746-51. (H35-17.2, FC, PubMed)

Ledbetter JA, Seaman WE, Tsu TT, Herzenberg LA. Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity. J Exp Med. 1981 Jun 1;153(6):1503-16.

Golstein P, Goridis C, Schmitt-Verhulst AM, Hayot B, Pierres A, van Agthoven A, Kaufmann Y, Eshhar Z, Pierres M. Lymphoid cell surface interaction structures detected using cytolysis-inhibiting monoclonal antibodies. Immunol Rev. 1982;68:5-42. Review.

Tarleton RL, Sun J, et al. 1994. Depletion of T-cell subpopulations results in exacerbation of myocarditis and parasitism in experimental Chagas' disease. Infect Immun. 62(5):1820-9. (H35-17.2, IHC frozen, PubMed)

Thoma-Uszynski S, Emoto M, and Kaufmann SH. 1997. CD8alphaalpha T cells in lesions of Listeria monocytogenes-infected beta2m-deficient mice. Microb Pathog. 23(2):101-6. (H35-17.2, IHC frozen, PubMed)

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Cat. 53-4031 Alexa Fluor® 488 Rat IgG2b Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.